抑癌基因CT—GalNAcT转染对结肠癌细胞COLO26的影响  

Influence of Transfection of Tumor Suppression Gene CT - GalNAcT on Co-lon Cancer Cell COLO26

作　　者：朱俊铭[1] 全家妩[1] 村松知成 北中千史 口野嘉幸 

机构地区：[1]武汉生物制品研究所 [2]日本国立癌中心研究所生物物理部

出　　处：《中国生物制品学杂志》2002年第3期151-154,共4页Chinese Journal of Biologicals

摘　　要：【摘 要】目的 了解抑癌基因 CT－CalNAcT（N－acetylgalactosamine Transferase）对小鼠结肠癌细胞 COLO26的作用。方法 将抑癌基因 CT－GalNAcT序列构建到带有报道基因 FLAG的质粒上，重组质粒 pFLA－G－GalN－AcTDNA在阳离子脂质体Effectene介导下转染，并分别与绿色荧光蛋白基因质粒pcDNA3EGEP和潮霉素抗性基因质粒pCEP4转染结肠癌细胞COLO26，观察瘤细胞的生长状况。裂解细胞提取蛋白进行 Western blotting。结果 瘤细胞变圆，粘附性下降，细胞生长受到抑制，死亡细胞增多。与 pcDNA3EGEP、pCEP4共转染及 Western blotting确定 CT－CalNAcT已经转染到COLO26细胞内。结论 抑癌基因CT－GalNAcT对结肠癌细胞具有生长抑制作用。[ Abstract] Objective To study on the influence of transfection of tumor suppression gene CT - GalN-AcT ( N - acetylalactosamine transferase) on colon cancer cell COLO26. Methods A recombinant plasmid pFLAG - CT - GalNAcT was constructed by inserting tumor suppression gene CT - GalNAcT into pFLAG vector and transformed to the strain JM 109 of E. coli. Being mediated by Effectene, a kind of cation liposome, the DNA of the recombinant plasmid was transfected alone to colon cancer cell COLO26 or co - transfected with plasmids pcDNASEGFP and pCEP4 respectively, and the growth of COL026 cells were observed. Protein was extracted from the cells and detected by Western blot. Results The cancer cells turned to round, and the adhesiveness of them decreased. The cell growth was significantly inhibited, while the counts of dead cells increased . Western blotting proved that CT - GalNAcT gene had been successfully transfected to COL026 cells. Conclusion Tumor suppression gene CT - GalNAcT showed significant inhibiting effect on the growth of colon cancer cell COLO26.

关 键 词：抑癌基因 CT-GalNAcT 转染 结肠癌细胞 

分 类 号：R735.35[医药卫生—肿瘤]