硝酸钠浓度对三角褐指藻(Phaeodactylum tricornutum)MACC/B226生长及脂肪酸组成的影响  

EFFECT OF NaNO_3 CONCENTRATIONS ON THE GROWTH AND FATTY ACID COMPOSITIONS OF Phaeodactylum tricornutum MACC/B226

作　　者：梁英[1] 麦康森[1] 孙世春[1] 于道德[1] 

机构地区：[1]青岛海洋大学海水养殖教育部重点实验室,266003

出　　处：《海洋科学》2002年第5期48-51,共4页Marine Sciences

基　　金：国家863高新技术发展计划项目863 819 02 01号;青岛海洋大学海水养殖教育部重点实验室研究报告389号

摘　　要：在温度为22±1℃ ,盐度为28,硝酸钠浓度分别为75,375,750,1125,1500,1875mg/L的条件下 ,用F/2培养基对青岛海洋大学微藻种质库保存的三角褐指藻(Phaeodactylumtricor-nutum)MACC/B226进行培养 ,测定了生长及脂肪酸组成。实验结果表明 ,在硝酸钠浓度为1125mg/L时 ,三角褐指藻相对生长率最高。在75～375mg/L之间二十碳五稀酸 (EPA)含量随着硝酸钠浓度的增加而增加 ,在375～1875mg/L之间EPA含量变化不大 ,在1875mg/L时达到最高值 (占总脂肪酸的12.6% )。Relative growth rate and fatty acid compositions of Phaeodactylum tricornutum UTEX640 were examined. The glgae were grown under different initial NaNO 3 concentrations (75, 375, 750, 1 125, 1 500 and 1 875 mg/L) and harvested at the late exponential phase. The culture temperature was 22±1 ℃; the salinity was adjusted to 28 by distilled water. The results showed that the optimum NaNO 3 concentration for the highest relative growth rate was 1 125 mg/L. The optimum NaNO 3 concentration for the highest EPA content was 1 875 mg/L.

关 键 词：硝酸钠浓度 三角褐指藻 生长 脂肪酸组成 相对生长率 微藻饲料 

分 类 号：S963.213[农业科学—水产养殖] Q949.2[农业科学—水产科学]