荧光假单胞菌株M18产PCA发酵条件研究  

STUDY ON FERMENTATION CONDITION FOR PCA PRODUCT BY Pseudomonas fluorescens M18

作　　者：罗鹏[1] 许煜泉[1] 张霞[1] 陈峰[1] 

机构地区：[1]上海交通大学生命科学技术学院

出　　处：《上海农业学报》2002年第2期66-71,共6页Acta Agriculturae Shanghai

基　　金：上海市科技发展基金项目 (995 4 130 2 8)

摘　　要：在摇瓶发酵条件下 ,研究了产吩嗪 1 羧酸 (phenazine 1 carboxylicacid ,PCA)的荧光假单胞菌M 18在各种碳源、氮源、盐类、温度、接种量下PCA的积累情况 ,确定了适合PCA分泌的最佳培养基 (1L)为 :葡萄糖 2 0 g ,肉胨 2 2 g ,KNO310 g ,NaCl 0 g ;最佳培养温度为 2 9℃、接种量为5 % ;最佳培养时间为 4 4h左右。在此条件下 ,PCA在发酵液中的浓度可达到 75 0mg/L 。Under the condition of shaking flask fermentation, the potential ability of phenazine 1 carboxylic acid (PCA) produced by Pseu domonas fluorescens M18 was studied at different carbon sources,nitrogen sourc es, content of salts, temperature and inoculation amounts. And the optimum cult ure condition suitable to PCA secretion was determined. Culture medium(1L) was 2 0g glucose, 22g meat peptone, 10g KNO 3, 0g NaCl; the inoculation amount was 5% ,the temperature was 29℃,and the culture time was about 44h. In this condition, the PCA concentration in fermentation liquor was as high as 750mg/L ,which coul d provide valuable reference for large scale fermentation.

关 键 词：荧光假单胞菌株 M18 PCA 发酵条件 抗生素 

分 类 号：S482.28[农业科学—农药学] TQ465[农业科学—植物保护]