K型小麦细胞质雄性不育系育性恢复基因的SSR分子标记分析  

Mapping of Fertility Restoring Gene for Aegilops kotschy Cytoplasmic Male Sterility in Wheat Using SSR Markers

作　　者：刘保申[1] 孙其信[2] 高庆荣[1] 孙兰珍[1] 解超杰[2] 李传友[1] 倪中福[2] 窦秉德[2] 魏艳玲[2] 

机构地区：[1]山东农业大学农学院,泰安271018 [2]中国农业大学植物遗传育种系,北京100094

出　　处：《中国农业科学》2002年第4期354-358,共5页Scientia Agricultura Sinica

基　　金：国家自然科学基金资助项目 (3 990 0 0 88)

摘　　要：以 (K冀 5 4 18A∥ 9112 89/LK783)三交F1分离群体的极端不育株和极端可育株分别建立保持池和恢复池 ,利用 79对SSR引物对两池间的多态性进行了研究。分析表明 ,6对SSR引物在两池间扩增出了稳定的多态性差异 ,在分离群体上验证结果表明 ,LK783的育性恢复基因与 4个SSR引物的扩增位点Xgwm11、Xgwm18、Xgwm2 6 4a和Xgwm 2 73有连锁关系 ,该育性恢复基因与Xgwm11、Xgwm18和Xgwm2 73的遗传距离为 6 .5 4± 4 .37cM ,与Xg wm2 6 4a的遗传距离为 5 .71± 4 .10cM ,这 4个引物可应用于K型小麦细胞质雄性不育系育性恢复基因的标记辅助选择。利用中国春缺体四体系和双端体系进一步将Xgwm11、Xgwm18、Xgwm2 6 4a和Xgwm2 73定位于 1BS ,说明LK783的育性恢复基因位于 1BS ,但它在 1BS上的相对位置与Rfv1有所不同 。LK783 was found to be a good fertility restorer for K type male sterility of wheat. Microsatellite markers were employed to map the major restoring gene in LK783. Maintainer and restorer DNA pools were established using the extreme sterile and fertile plants among (KJ5418A∥911289/LK783)F 1 population, respectively. Seventy nine sets of SSR primers were screened for polymorphism between the two pools, 6 of which were found polymorphic. Linkage analysis showed that Xgwm11,Xgwm18,Xgwm264a and Xgwm273 were linked to the restoring gene in LK783, while Xgwm11,Xgwm18 and Xgwm273 were co segregated. The distance between the Rf gene in LK783 and the three co segregated markers was 6.54±4.37cM, the distance between Rf gene and Xgwm264a was 5.71±4.10cM. The four SSR markers were located on chromosome 1BS by amplifying the DNA of nulli tetrasomics and ditelosomics of CS with the 4 sets of primers, indicating that the restoring gene in LK783 was located on 1BS, but the relative location of the gene was different from Rfv1, the allelism of the two genes should be further investigated. The breeding for new fertility restorer lines of K type cytoplasmic male sterility in wheat would be facilitated by using the four polymorphic markers.

关 键 词：K型小麦 细胞质雄性不育系 育性恢复基因 SSR分子标记 微卫星 

分 类 号：S512.1[农业科学—作物学]