应用mRNA差异显示法初步研究肺癌相关基因  

Preliminary study on related genes in lung cancer tissues associated with residential radon exposure

作　　者：夏英[1] 杨梅英[2] 冯顺治 张守志[1] 叶常青[2] 

机构地区：[1]卫生部工业卫生实验所,北京100088 [2]北京放射医学研究所 [3]甘肃省庆阳地区人民医院

出　　处：《中华放射医学与防护杂志》2002年第2期83-85,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金资助项目 (3 9970 2 3 5 )

摘　　要：目的　研究高氡暴露地区居民肺癌组织中相关未知基因 ,探讨肺癌发生的机理。方法　应用mRNA差异显示方法从同一例肺癌和正常肺组织中选出有表达差异的基因片段 ,经反向NorthernBlot杂交 ,将肺癌组织表达呈阳性的片段而正常肺组织呈阴性的片段进行克隆测序。结果7条差异片段经克隆测序后发现 ,其中NA7与GenbankEST中的AI2 0 86 6 7序列同源性达 95 %以上 ,NG2 与 5条基因序列同源性高达 98% ,而CA1 可能是一个新的差异基因片段。结论　从高氡暴露地区居民肺癌和正常组织中获得 3条可能与肿瘤相关的基因片段。Objective\ To search for the related genes in lung cancer tissues associated with residential radon exposure. Methods\ Gene fragments expressed differentially in lung cancer and normal lung tissues were discovered by differential display method.Then reverse Northern Blot hybridization,the positive reaction fragments in lung cancer and negative fragments in normal lung tissue were determined. Results\ One of 7 targeted sequences coded NA\-7 had up to 95% of homology with AI208667 in EST of Genbank,second one NG\-2 had up to 98% of homology with five fragment sequences.The remained one CA\-1 may be a new gene fragment. Conclusion 3 gene fragments were discovered from lung cancer and normal tissue of high radon exposure resident.\;

关 键 词：MRNA 差异显示法 研究 肺癌 相关基因 

分 类 号：R734.2[医药卫生—肿瘤]