c-myc反义寡核苷酸对黏液表皮样癌MEC-1细胞超微结构的影响  

Effects of antisense oligodeoxynucleotides of c-myc gene on ultrastructure of mucoepidermoid carcinoma MEC-1 cells

作　　者：王石光[1] 司徒镇强[2] 朱光第[1] 

机构地区：[1]广州军区广州总医院口腔科,广州510010 [2]第四军医大学口腔医学院,西安710032

出　　处：《广东医学》2002年第5期456-458,共3页Guangdong Medical Journal

摘　　要：目的　探讨c -myc反义寡核苷酸 (ASODN)对人黏液表皮样癌MEC -1细胞超微结构的作用特点。方法　将实验分成两组 ,处理组用 10 μmol/L 的c -mycASODN作用于细胞 72h ;对照组细胞未经处理 ,分别采用扫描电镜(SEM)和透射电镜 (TEM)观察各组细胞超微结构的形态变化。结果　SEM观察经c -mycASODN作用的黏液表皮样癌MEC -1细胞微绒毛明显减少且缺乏立体感而呈平铺状 ;TEM观察处理组细胞亦可见表面的微绒毛减少 ,且胞浆中有明显的空泡形成及线粒体肿胀等。对照组细胞超微结构无此类变化。结论　c -myc反义寡核苷酸对人黏液表皮样癌MECObjective To investigate the effects of c-myc antisense oligodeoxynucleotides(ASODN) on the ultrastructure of human mucoepidermoid carcinoma MEC-1 cells. Methods MEC-1 cells, treated with and without 10 μmol/L of c-myc ASODN, were served as c-myc ASODN group and control group, respectively. The ultrastructural changes of MEC-1 cells were observed under scanning electromicroscope(SEM) and transmission electromicroscope(TEM) after 72 h . Results Significant decrease in the number of MEC-1 cell microvilli was observed under SEM and TEM in c-myc ASODN treated group. Many vacuoles and swollen mitochondria were also found in the cytoplasma under TEM. Conclusion c-myc ASODN can decrease the malignant degree of the human mucoepidermoid carcinoma MEC-1 cells.

关 键 词：寡脱氧核苷酸 黏液表皮样癌 超微结构 c-myc ASODN MEC-1细胞 

分 类 号：R739.85[医药卫生—肿瘤] R730.261[医药卫生—临床医学]