端粒酶活性在人膀胱肿瘤组织中表达的临床意义  

The clinical significance of telomerase activity and PCNA expression in human bladder carcinoma

作　　者：钟惟德[1] 胡建波[1] 蔡岳斌[1] 曾广翘[1] 戴奇山[1] 肖聪勤[1] 王良圣[1] 魏鸿蔼[1] 高劲松[2] 刘建康[3] 黄少华[3] 

机构地区：[1]广州医学院附属第一人民医院泌尿外科,广州510180 [2]中山医科大学基因诊断中心 [3]广州医学院组胚教研室

出　　处：《临床泌尿外科杂志》2002年第5期231-232,共2页Journal of Clinical Urology

基　　金：广东省科委重点攻关课题 [粤计科字 (2 0 0 0 ) 2 6 1]

摘　　要：目的 :探讨不同临床病理类型的膀胱肿瘤及癌旁组织端粒酶活性表达及临床意义。方法 :以改良TRAP法测定 91例膀胱肿瘤组织标本端粒酶活性表达。结果 :83例膀胱移行细胞癌组织中 78例检测到端粒酶活性 ,阳性率为 94%,其对应的癌旁组织也有 14%的检出率。 8例膀胱乳头状瘤组织中 4例检测到端粒酶活性 ,阳性率为 5 0 %,其对应的癌旁组织检出率为 12 %。端粒酶活性在不同临床病理类型的膀胱肿瘤及癌旁组织中表达差异无显著性意义 (P >0 .0 5 )。结论 :膀胱肿瘤及癌旁组织端粒酶活性的检测对肿瘤的诊断有重要意义。Purpose: To study the clinical significance of telomerase activity in different types of bladder carcinoma and pericarcinomatous tissue. Method:The telomerase activity and PCNA expression were determined by modified TRAP in 91 cases of bladder carcinoma. Result:78 of 83 cases of bladder transitional cell carcinoma were positive in the test (94%) and with 14% of detectable findings in pericarcinomatous tissues. 4 of 8 cases of bladder papilloma were positive (50%) and with 12% of detectable findings in pericarcinomatous tissue. No significant difference was noted in expression of telomerase activity in various pathological types of bladder carcinoma (P> 0.05 ). Conclusion:Determination of telomerase in bladder carcinoma and pericarcinomatous tissue is effective for treatment and prognosis estimation.

关 键 词：膀胱肿瘤 端粒酶 聚合酶链反应 TRAP法 端粒酶活性 诊断 膀胱癌 

分 类 号：R737.14[医药卫生—肿瘤]