胆管癌细胞层粘连蛋白受体表达下调对蛋白水解酶MMP-2、MMP-9 mRNA表达的影响  

MODULATION OF ANTISENSE PHOSPHOROTHIOATE OLIGODEXYNUCLEOTIDES OF LAMININ RECEPTOR ON MMP-2、MMP-9 mRNA EXPRESSION IN HUMAN BILE DUCT CARCINOMA

作　　者：陈长宏[1] 王曙光[1] 李大江[1] 刘利[1] 

机构地区：[1]第三军医大学西南医院

出　　处：《解放军医学杂志》2002年第5期423-425,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金资助课题 (编号 39870 830 )

摘　　要：研究层粘连蛋白受体 (lamininreceptor,LNR)反义寡核苷酸硫代修饰片段 (AS OD)对人胆管癌QBC939细胞MMP 2、MMP 9基因表达的调节。应用逆转录聚合酶链反应 (RT PCR)方法分析 12 μmol/L浓度的LNRAS OD在作用 72h后对QBC939细胞MMP 2、MMP 9mRNA表达水平的影响。结果显示 ,LNRAS OD能明显抑制人胆管癌细胞表达MMP 2、MMP 9的作用 ,与正常对照相比 ,其MMP 2、MMP 9mRNA相对丰度分别下降了 33 2 %和 2 3 9% (P <0 0 5 )。研究表明 ,LNRAS OD是胆管癌细胞表达MMP 2和MMP 9基因的调节剂 ,能抑制胆管癌细胞MMP 2、MMP 9基因的表达。This study was designed to investigate modulation of phosphorothioate oligodexynucleotides of laminin receptor on MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Treated with a concentration of 12μmol/L LNR AS OD in cuture medium, the relative MMP 2 and MMP 9 mRNA level at 72h in human bile duct carcinoma cells was evaluated by reverse transcriptase polymerase chain reaction. The results showed that the expression of MMP 2 and MMP 9 dropped obviously by AS OD group as compared with control group. The relative expression level of MMP 2 and MMP 9 gene mRNA decreased about 33 2% and 23 9% respectively. It is suggested that LNR AS OD is the regulator of MMP 2 and MMP 9 gene expression. It can decrease MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells. Understading the regulation of MMP 2 and MMP 9 gene expression in human bile duct carcinoma cells might contribute to the development of a new preventive and theraputic strategy for tumor invasiveness.

关 键 词：受体 层粘连蛋白 寡核苷酸类 胆管癌 基质金属蛋白酶 肿瘤转移 MMP-2 MMP-9 mRNA 

分 类 号：R735.8[医药卫生—肿瘤] R73－37[医药卫生—临床医学]