外源性p27与GRC-1细胞端粒酶活性及细胞凋亡关系的实验研究  

THE EXPERIMENTAL RESEARCH OF THE RELATIONSHIP BETWEEN TELOMERASE ACTIVITY AND APOPTOSIS OF GRC-1 CELLS WITH EXOGENOUS p27 PROTEIN EXPRESSION

作　　者：张运涛[1] 刘凡[1] 姜茹[2] 谷仲平[3] 汪涌[1] 张顺[1] 刘荣福[1] 李玉梅[1] 

机构地区：[1]第四军医大学唐都医院泌尿外科,西安710038 [2]第四军医大学基础部化学教研室 [3]第四军医大学唐都医院胸外科

出　　处：《中国现代医学杂志》2002年第9期3-5,共3页China Journal of Modern Medicine

摘　　要：目的 :观察p2 7蛋白表达水平与肾癌GRC - 1细胞端粒酶活性的关系及对其诱导凋亡的作用。方法 :采用脂质体介导的方法将 p2 7质粒导入GRC - 1细胞中 ,应用TRAP -PCR -ELISA、电镜及Tunel等技术检测外源性 p2 7蛋白对GRC - 1细胞端粒酶活性、细胞凋亡的影响。结果 :转染 p2 7质粒的GRC - 1细胞端粒酶活性显著下降 ,伴有细胞凋亡、细胞生长停滞。结论 :外源性p2 7基因的表达可使GRC - 1细胞端粒酶活性降低 ,细胞生长停滞 ,诱发细胞凋亡。提示细胞端粒酶活性的改变与细胞周期的调控密切相关。Objective:To observe the effect of exogenous p27expression on GRC-1 cells and to investigate the relationship between p27 expression and cellular telomerase activity.Methods:p27 cDNA was introduced into GRC-1 cells by using lipofectin mediation; By means of TRAP, electromicroscope and Tunel,the changes of GRC-1 cells in telomerase activity and cell apoptosis were detected.Results:A significant association was observed between increased expression of p27 and decreased telomerase activity. Compared with control,GRC-1/pCMV-p27 presented a significant drop in telomerase activity expression,accompanied by remarkable apoptosis and growth arrest.Conclusions:Exogenous p27 protein over-expression promotes apoptosis of GRC-1 cells and reduces telomerase activity and can inhibit cell proliferation.These indicate that controlling the cell cycle correlated with the changes of telomerase activity.

关 键 词：P27蛋白 端粒酶 细胞凋亡 肾癌 

分 类 号：R737.11[医药卫生—肿瘤]