清开灵对谷氨酸神经毒性脑损伤脑组织c-fos基因表达的影响  

Effects of Qingkailing Injection on c-fos Gene Expression in Treating Neurotoxic Brain Injury Induced by Glutamate

作　　者：岳少杰[1] 罗自强[2] 冯德云[3] 伍赶球[4] 

机构地区：[1]中南大学湘雅医院儿科,长沙410078 [2]中南大学湘雅医学院生理学教研室,长沙410078 [3]中南大学湘雅医学院病理学教研室,长沙410078 [4]中南大学湘雅医学院组织胚胎学教研室,长沙410078

出　　处：《北京中医药大学学报》2002年第2期27-30,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助课题 (No .3970 0 193)

摘　　要：采用免疫组织化学方法及放射配体结合分析法 ,观察清开灵及侧脑室注射c fos反义寡核苷酸阻断c fos基因表达后脑组织蛋白激酶C(PKC)表达量和Glu的N_甲基_D_天门冬氨酸 (NM DA)受体的数目、亲和力变化的影响。结果 :清开灵可有效地抑制Glu所致的脑组织c fos蛋白及PKC蛋白的表达增强 ,并下调Glu的NMDA受体数目 ;而c fos反义及正义寡核苷酸对Glu的NMDA受体数目及Glu所致的脑组织PKC蛋白表达增多均无明显影响。结论 :清开灵抗Glu神经毒性的脑保护作用的机制与其抑制cThe effects of Qingkailing Injection (QI) on c fos gene expression in relieving neurotoxic brain injury induced by glutamate were studied in rats by the immunohistochemical method and radioligand binding assay. The effects of QI and of the blockage of c fos gene expression by the right ventricle injection of c fos antisense oligonucleotide on the amount of cerebral protein kinase C (PKC) expression, and on the amount and affinity of N methyl D aspartic acid (NMDA) receptor of glutamate were also studied by the methods as same as the above mentioned. The results showed that QI could effectively inhibit the increase of the expression of cerebral c fos protein and PKC protein induced by glutamate, and lower the amount of NMDA receptor of glutamate; while both c fos antisense oligonucleotide and c fos sense oligonucleotide had no marked effects on the amount of NMDA receptor of glutamate and the increase of the expression of cerebral PKC protein induced by glutamate. The results suggest that the mechanism of the protective effects of QI against glutamate induced cerebral injury is related to its inhibiting the expression of c fos protein and PKC protein and lowering the amount of NMDA receptor.

关 键 词：谷氨酸盐类 脑损伤 C-FOS基因 蛋白激酶C 免疫组织化学 清开灵注射液 

分 类 号：R285.5[医药卫生—中药学]