耐氨固氮的催娩克氏菌的高密度培养研究  

STUDY ON HIGH-DENSITY CULTURE OF AMMONIUM-RESISTANT N_2-FIXING BACTERIUM KLEBSIELLA OXYTOCA

作　　者：卢秋雁[1] 谢小保[1] 朱红惠[1] 邱晓颖[1] 丘明祺[1] 丘元盛[1] 

机构地区：[1]广东省微生物研究所,广州510070

出　　处：《微生物学通报》2002年第2期42-45,共4页Microbiology China

基　　金：国家863计划资助项目(No.101-03-04-01)~~

摘　　要：　根据耐氨固氮型催娩克氏菌的特点,对其发酵工艺进行了研究。确定了以葡萄糖为碳源,合理搭配适量的氮源及无机盐作为高密度培养的基础培养基;在培养的中后期,添加葡萄糖补充碳源,流加氨水补充氮源,同时将pH值稳定在6.5～6.8之间,通过控制适当的通气量、搅拌速度以维持适当的溶氧水平。培养终止时,NG13/pMC73A的菌数达到600～700×108cfu/mL,与原有工艺相比,菌数提高十倍以上,而培养周期基本相同。Based on the physiological properties of ammonium-resistant N2-fixingbacterium ( Klebsiella oxytoca NG13/pMC73A), the fermentation technology of it was studied. The basic medium of high-density culture was established, with glucose as carbon source coupled with appropriate nitrogen source and inorganic salts. At the middle and late phase of culture, glucose and ammonia were added to supply carbon source and nitrogen source, stabilizing the pHat 6.5 ~ 6.8. Optimal level of dissolved oxygen was kept by controlling aeration and stirring rate. Bacterium number of Klebsiella oxytoca NG13/pMC73A reached 600 ~ 700 x 10s cfu/mL at the end of culture. Compared with previous technology, bacterium number was increased by more than ten-fold with a comparable culture period.

关 键 词：催娩克氏菌 高密度培养 固氮菌 耐氨固氮 

分 类 号：TQ920[轻工技术与工程—发酵工程]