左侧视网膜剥夺鸽右视盖差异表达基因的克隆与鉴定  

IDENTIFICATION AND CLONING OF THE DIFFERENTIALLY EXPRESSED GENES IN RIGHT OPTIC TECTA OF PIGEONS WITH THEIR LEFT-RETINA REMOVED

作　　者：吕立夏[1] 徐磊[1] 杨翠香[1] 李学礼[1] 王尧[1] 

机构地区：[1]同济大学基础医学院生化教研室,上海200070

出　　处：《神经解剖学杂志》2002年第1期51-54,共4页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金 (No. 3 9770 2 44 )资助项目

摘　　要：采用抑制消减杂交技术构建左侧视网膜剥夺后右视盖基因差异表达的文库 ,研究在无光刺激的条件下视盖发育受阻的分子机制。随机测序 2 1个序列 ,6个序列经反 Northern证实为真阳性 ,3个序列未发现明确的同源序列 ,另外 3个分别与 homosapiens SH3 GL2、G.domesticaus、 transthyretin有较高的同源性。 2 1个序列中 ,11个基因片段被分配序列号。Using suppression subtractive hybridization technique, the differentially expressed cDNA library for right optic tecta of pigeons was constructed to investigate the molecular mechanism for visual development of the optic tecta without light stimulation. Twenty one of expressed sequenced tags (ESTs) were sequenced randomly. six of EST were proved true positive by reverse Northern Blotting, three of them were found no high homology with genes in Genbank, the rest sharing higher homology with homo sapiens SH3GL2, G.domesticaus, transthyretin. Eleven of ESTs were assigned Accession Number by Genbank. Our data provided useful clues for studying the development of visual system.

关 键 词：左侧视网膜剥夺 鸽 右视盖 差异表达基因 克隆 鉴定 抑制消减杂交 表达序列标签 

分 类 号：R346[医药卫生—基础医学]