神经保护剂的鸡尾酒疗法对大鼠局灶性脑缺血后梗死体积和神经元凋亡及bcl-2蛋白表达的影响  

Effect of neuroprotectant combined with cocktail on focal cerebral ischemia in rats

作　　者：潘殿卿[1] 李志梅[1] 

机构地区：[1]哈励逊国际和平医院神经内科,河北衡水053000

出　　处：《中华神经科杂志》2002年第2期74-78,共5页Chinese Journal of Neurology

摘　　要：目的　探讨不同类型神经保护剂联合应用 ,即鸡尾酒疗法 (cocktail)是否较单一神经保护剂对局灶性脑缺血有更好的保护作用。方法　采用线栓法制作大鼠大脑中动脉闭塞模型 (MCAO) ,分为 1,6 二磷酸果糖 (FDP)组、MK 80 1组、N 乙酰半胱氨酸 (NAC)组、cocktail组和对照组 ,观察缺血后6h和 2 4h梗死体积、神经元凋亡和抗凋亡蛋白bcl 2表达的变化。结果　缺血后 6h和 2 4h ,cocktail组梗死体积减小 ,神经元凋亡减少 ,bcl 2表达增多 ,与单一用药各组相比有显著性差异。结论　神经保护剂cocktail疗法对鼠脑组织缺血的保护作用较单一用药明显增强。Objective To investigate whether cocktailtherapy combined with of neuroprotectants may have more advantages over single agents in treating focal cerebral ischemic cascade Methods Use suture occlusion technique, the right middle cerebral artery in rats was occluded Tirty minutes later,Fructose 1,6 diphosphate(FDP)(50 mg/kg, n =20), MK 801(1 mg/kg, n =20) and N acetylcystein(NAC)(150 mg/kg, n =20) were singly or combinantly infused intraperitoneally At the same time the cocktail treated group ( n =20) were infused the above agents combinationtly and the control group ( n =20) were infused normal saline intraperitoneally. six and 24 hours after focal cerebral ischemia,the animals were weighted and neurologically assessed on 5 point scale The animals were killed; brain were stained with 2,3,5 triphenylte trazolinm chloride for assessment of the volume of infarction, and then embedden onto slides with paraffin for morphological assessment and terminal transferase dUTP nick ending labeling(TUNEL) were carried out for apoptosis and immunohistochemistry were carried out to investigate the changes in bcl 2 Results All neuroprotectants decreased the volume of infarction( P <0 05, F test),while cocktail treated group showed more distinct decrease than other groups( P <0 05, F test) FDP treated group did not decrease the apoptosis of the neurons and did not increase the bcl 2 expression as well MK 801 treated group,NAC treated group and cocktail treated group significantly decreased the apoptosis of the neurons and increased the bcl 2 expression ( P <0 05, F test) With cocktail treated group showing more distinct effect( P <0 05, F test) Conclusion Cocktail may be more effective than single neuroprotectant in this modle

关 键 词：鸡尾酒疗法 大鼠 梗死体积 神经元凋亡 BCL-2蛋白表达 脑缺血 神经保护药 药物疗法 

分 类 号：R743.3[医药卫生—神经病学与精神病学]