肺癌组织中端粒酶基因与端粒酶活性关系的研究  

Study on the relationship between telomerase gene and telomerase activity in lung cancer tissue

作　　者：陈蔚蔚[1] 熊小熊[1] 周宏远[1] 周清华[1] 

机构地区：[1]四川大学华西医学中心华西医院(原华西医科大学附属第一医院)肿瘤中心,肿瘤研究所,成都610041

出　　处：《中国肺癌杂志》2002年第1期31-34,共4页Chinese Journal of Lung Cancer

基　　金：国家自然科学基金 ( 39570 392 )资助

摘　　要：目的　探讨肺癌组织中端粒酶基因hTR、hTERT与端粒酶活性的表达是否与肺癌的发生发展有关 ,深入了解端粒酶基因对端粒酶活性的调控是在基因水平还是在转录水平。方法　采用TRAP PCR和RT PCR方法分别检测 68例肺癌组织及相应癌旁肺组织中端粒酶活性、端粒酶基因hTR和hTERT的表达。结果　 68例肺癌组织中端粒酶阳性率为 79.4 % (54/ 68) ,hTR阳性率为 98.5% (67/ 68) ,hTERT阳性率为 91 .2 % (62 /68)。68例癌旁组织中无一例表达端粒酶阳性 ,但大多数癌旁组织均表达hTR(62 / 68,91 .2 % ) ,仅 7例hTERT表达阳性 (1 0 .3 % )。与hTR相比 ,hTERT同端粒酶具有更高的一致性 ,其一致率为 89.0 % (1 2 1 / 1 36) ,而hTR与端粒酶的一致率为 43 .4 % (59/ 1 36)。结论　端粒酶的活性可能在肺癌发生发展中起重要的作用。hTR与hTERT可能是在转录后或翻译后水平对端粒酶进行调控的。Objective To investigate the relationship between the telomerase activity and telomerase component expression in lung cancer, and to explore whether telomerase activity is regulated at the gene or transcriptional level. Methods Expression of the hTR and hTERT were detected in 68 human lung cancer tissues and responsive adjacent non neoplastic lung tissues by semi quantitative reverse transcription polymerase chain reaction. Telomerase activity was detected by telomeric repeat amplification protocol. Results In 68 lung cancer tissues, the positive rate of the telomerase activity, hTR and hTERT expression were 79.4% (54/68), 98.5% (67/68) and 91.2% (62/68) respectively. Whereas most adjacent non neoplastic lung tissues expressed hTR (62/68, 91.2%) also, hTERT was detected in only 7 (10.3%) normal lung tissues and no telomerase activity were detected in the 68 non neoplastic lung cancer tissues. As compared with hTR, hTERT expression was closely related to telomerase activity. The concordance was 89.0% (121/136), whereas the concordance between telomerase and hTR was 43.4% (59/136). Conclusion The results suggest that telomerase may play an important role in tumorigenesis of lung cancer. Telomerase activity may be regulated in transcription level or translation level.

关 键 词：端粒酶基因 端粒酶活性 端粒酶 HTR HTERT 肺肿瘤 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]