端粒酶活性在肺癌患者痰液脱落细胞中表达的临床价值研究  

Study on the clinical value of telomerase activity in exfoliated cells of sputum from lung cancer patients

作　　者：王金亮[1] 王嘉祺[2] 安真光[3] 居军[4] 

机构地区：[1]兰州医学院 [2]甘肃省人民医院 [3]兰州医学院附属第二医院 [4]甘肃省临床检验中心

出　　处：《中国肺癌杂志》2002年第1期38-40,共3页Chinese Journal of Lung Cancer

摘　　要：目的　探讨端粒酶活性在肺癌患者痰液中表达的临床意义。方法　运用端粒酶PCR 酶联免疫吸附测定法 (ELISA)检测了 45例肺癌、2 5例肺部良性疾病及 2例转移性肺癌患者痰液标本中的端粒酶活性。结果　肺癌患者痰液标本中端粒酶活性阳性率为 55.6 % (2 5/ 4 5) ,肺部良性疾病患者的痰液标本中端粒酶活性阳性率为 2 8.0 % (7/ 2 5) ,二者经统计学处理有显著性差异 (χ2 =5 .62 8,P <0 .0 5)。结论　检测肺癌患者痰液中端粒酶活性检测有助于肺部良恶性疾病的诊断和鉴别诊断。Objective To investigate the clinical value of the detection of telomerase activity in the sputum specimens of lung cancer. Methods The telomerase activity in sputum specimens was detected in 45 case of lung cancer and 25 cases of benign pulmonary diseases and 2 cases of metastatic carcinoma of the lung by PCR ELISA assay. Results Twenty five sputum specimens from lung cancer patients were positive for telomerase activity, and the sensitivity was 55.6% (25/45); seven sputum specimens from benign pulmonary diseases were positive, and the sensitivity was 28.0% (7/25). Significant difference was existed between the two groups (χ 2=5.628, P<0.05). Conclusion Detection of telomerase activity in the sputum specimens may be helpful to differentiate malignant from benign pulmonary diseases.

关 键 词：肺肿癌 痰标本 端粒酶活性 TRAP-PCR-ELISA法 肺癌 诊断 

分 类 号：R734.2[医药卫生—肿瘤]