工程菌表达马铃薯钙调素(PCM6)的抗体制备及鉴定  

Preparation and Identification of Antibody Against Potato CaM(PCM6) Purified from the Engineering Bacteria

作　　者：白娟[1] 毛国红[1] 郭毅[1] 王志玲[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016

出　　处：《河北师范大学学报（自然科学版）》2002年第3期292-294,共3页Journal of Hebei Normal University：Natural Science

基　　金：国家自然科学基金重点资助项目 ( 39730 2 30 )

摘　　要：PCM 6是马铃薯钙调素亚型中与其他植物钙调素在氨基酸序列上最为保守的一种 .利用大肠杆菌表达系统 ,表达并纯化了这种蛋白 .以纯化的重组马铃薯 Ca M( PCM6 )蛋白为抗原 ,免疫新西兰兔 ,获得了高效价 ( 1∶ 1 6 0 0 0 )抗体 .免疫印迹检测表明 ,制备的抗体与植物钙调素有特异性的免疫交叉反应 .PCM6 is one of the most conversed CaM isoforms in plants.The protein (PCM6) was expressed in the Engineering Bacteria and was purified.Purified recombinant CaM (PCM6) was used as antigen to immune rabbits and antibody was obtained.The titer of antibody is 1∶16000 by ELISA analysis.Western blotting showed that the antibody specifically cross-reacted with CaM from plants.

关 键 词：工程菌 PCM6 抗体 制备 鉴定 钙调素 马铃薯 免疫交叉反应 基因表达 氨基酸序列 抗原 

分 类 号：Q942.6[生物学—植物学] R392.11[医药卫生—免疫学]