RA/IFN-γ协同诱导C6胶质瘤细胞分化的研究  

The synergistic modulation effects of RA/IFN-γ on differentiation of C6 glioma cells

作　　者：钟春龙[1] 罗其中[1] 王勇[1] 邱永明[1] 徐纪文[1] 

机构地区：[1]上海第二医科大学附属仁济医院神经外科,上海200001

出　　处：《中华神经外科疾病研究杂志》2002年第2期157-159,共3页Chinese Journal of Neurosurgical Disease Research

基　　金：上海市科学技术发展基金资助项目(014119014)

摘　　要：目的 探索诱导分化新策略对脑胶质瘤的潜在治疗作用。方法 观察协同诱导分化处理前后C6胶质瘤细胞形态改变并应用免疫组化染色法检测其胶质纤维酸性蛋白（GFAP）表达变化。结果 维甲酸（RA）及干扰素-γ（IFN-γ）诱导分化可使C6细胞出现进一步向星形细胞分化的特异性形态学改变;空白对照组细胞大多数呈GFAP弱阳性反应;而RA组与RA/IFN-γ组细胞的GFAP表达量显著增加（P1,P2<0.01）,RA/IFN-γ组与单用RA组或IFN-γ组相比增加更显著（P<0.01）。结论 协同诱导分化疗法能促使胶质瘤细胞进一步成熟分化。Objective To study the feasibility of differentiation therapy in the treatment of malignant gliomas. Methods Synergistic modulation effects of RA and IFN-γ on the morphological appearances of cultured C6 glioma cells were observed and changes in GFAP expression were studied by SP immunohistochemical staining. Results More differentiated morphological appearances resembling normal astrocytes were observed after RA/IFN-γ differentiation therapy; most of the untreated cells showed low GFAP expression, but RA or RA/ IFN-γ therapy significantly elevated GFAP expression (P1, P2<0.01); The elevated expression of GFAP in RA/ IFN-γ treated cells was significantly different from that in cells treated with RA or IFN-γ alone (P < 0. 01). Conclusion The synergistic differentiation therapy can induce the poorly differentiated immature glioma cells to grow into well-differentiated cells.

关 键 词：神经胶质瘤 维甲酸 干扰素-Γ 脑胶质瘤 诱导分化疗法 

分 类 号：R739.410.5[医药卫生—肿瘤]