TCR Vβ8+IL-2/pcDNA3.1亚克隆重组质粒的构建  

Construction of recombinant plasmid of TCR Vβ8+IL-2/pcDNA3. 1

作　　者：张明智[1] 李继昌[2] 董子明[3] 李学民[4] 宗红 

机构地区：[1]郑州大学第一附属医院肿瘤科,郑州450052 [2]郑州大学第一附属医院消化内科,郑州450052 [3]郑州大学基础医学院病理生理学教研室,郑州450052 [4]郑州大学第一附属医院检验科,郑州450052 [5]郑州市第四人民医院肿瘤科,郑州450052

出　　处：《郑州大学学报（医学版）》2002年第3期277-280,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：博士基金资助课题

摘　　要：目的:构建TCR Vβ8 ＋IL－2/pcDNA3.1亚克隆重组质粒。方法:先将TCR Vβ8克隆人克隆载体pUC19,分别酶切 IL－2/pUC19和 TCR Vβ8/pUC19,将 IL－2连接到TCR Vβ8/pUC19重组质粒上,然后将 TCRVβ8＋ IL－2基因从 TCR Vβ8+IL－2/pUC19重组质粒上酶切下,克隆入pcDNA3．1表达载体上。结果和结论:经测序证实成功地构建了 TCR Vβ8＋IL－2/pcDNA3．l亚克隆重组质粒。Aim: To construct the subclone recornbinant plasmid of TCR Vβ8 IL-2/pcDNA3. 1. Methods:Gene of TCR Vβ8 was cloned into the cloning vector pUC19 ;IL-2/pUC-19 and TCR Vβ8/pUC-19 were cleaved with endonu-clease, respectively; gene of IL-2 was linked to the recornbinant plasmid of TCR Vβ8/pUC-19. Then gene of TCR Vβ8 + IL-2 was cleaved with endonuclease from the recornbinant plasmid of TCR Vβ8 + IL-2/pUC-19 and cloned into expressing vector pcDNA3. 1. Results and Conclusion: The subclone recornbinant plasmids of TCR Vβ8 + IL-2/pcD-NA3. 1 had been constructed and identified by sequencing.

关 键 词：淋巴瘤 TCR IL-2 亚克隆 重组质粒 

分 类 号：R733.4[医药卫生—肿瘤] R318.5[医药卫生—临床医学]