细胞角蛋白CK19在口腔粘膜癌变过程中的变化  

The changes of cytokeratin 19 during oral carcinogenesis

作　　者：聂敏海[1] 钟利[2] 曾光明[3] 李秉琦[3] 

机构地区：[1]泸州医学院附属医院口腔内科,四川646000 [2]泸州医学院附属医院传染科,四川646000 [3]四川大学华西口腔医学院口腔内科

出　　处：《中华口腔医学杂志》2002年第3期187-190,W003,共5页Chinese Journal of Stomatology

基　　金：国家自然科学基金 (3 0 0 70 815 );四川省卫生厅科研基金 (960 0 40 )资助项目

摘　　要：目的　探讨口腔粘膜癌变过程中CK19的变化。方法　收集正常口腔粘膜、上皮单纯增生、上皮异常增生和口腔鳞癌活检标本共 5 3例 ,用免疫组化、电泳和Western杂交方法研究上皮中CK19的表达。结果　CK19表达于有上皮异常增生的复层鳞状上皮基底上层和口腔鳞癌尤其是低分化鳞癌的癌细胞中。随病变程度加重 ,CK19表达的阳性率、表达强度和占细胞角蛋白的构成比显著增加。结论　CK19表达于口腔粘膜上皮的基底上层可作为口腔癌前病变的辅助标志 。Objective To investigate the changes of cytokeratin 19 during oral carcinogenesis. Methods 53 specimens including normal oral mucosa, oral epithelial hyperplasia, oral epithelial dysplasia and oral squamous cell carcinoma were investigated by immunohistochemistry, SDS PAGE and Western blotting. Results CK19 was detectable in suprabasal cell layers in epithelial dysplasia and in oral cancer, especially in poor differentiated cancerous cells. With the lesions getting worse, the positive rate, the intensity and the constituent ratio of CK19 raised significantly. Conclusions The results suggest that the CK19 expression in suprabasal cell layers of oral mucosa can be used as a marker of diagnosis of oral precancerous lesions and CK19 expression is the initial events during oral carcinogenesis.

关 键 词：癌前病变 免疫组织化学 电泳 Western杂交法 细胞角蛋白CK19 口腔粘膜癌 

分 类 号：R739.8[医药卫生—肿瘤]