人血清白蛋白对人牙龈上皮细胞粘附作用的影响  

Effect of human serum albumin on cell attachment of human gingival epithelial cells

作　　者：刘宇[1] 施生根[2] 宋应亮[3] 林松杉[1] 郭航[1] 章禾[1] 

机构地区：[1]海军总医院口腔科,北京100037 [2]解放军第306医院口腔科 [3]第四军医大学口腔医学院修复科

出　　处：《中华口腔医学杂志》2002年第3期197-199,W005,共4页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 (3 9870 2 19;3 970 0 164 )

摘　　要：目的　探讨人血清白蛋白 (humanserumalbumin ,HSA)对人牙龈上皮细胞 (humangingivalepithelialcells ,HGE)粘附作用的影响。方法　使用角化细胞无血清培养基分离酶原代培养HGE ,并用广谱细胞角蛋白单抗做细胞鉴定 ,细胞接种 2 4h内用MTT法连续观测HSA聚苯乙烯表面预孵育组及培养基含HSA组HGE的粘附 ,在含HSA培养基中观测HGE的生长曲线。结果　HGE免疫组化染色阳性 ;HSA预孵育组 8h以内粘附的细胞数量显著少于培养基含HSA组及对照组 ,10～ 2 4h两实验组与对照组间差异无显著性 ;生长曲线中各时间点细胞的数量 ,实验组与对照组相比差异无显著性。结论　HSA预孵育于聚苯乙烯表面 。Objective To investigate the effect of human serum albumin(HSA)on cell attachment of human gingival epithelial cells(HGE). Methods HGE were primary cultured with keratinocyte serum free medium(KSFM) and dispase.The cultured cells were immunohistochemically stained by monoclonal anti pan cytokeratin.MTT test was employed to investigate the influence of HSA on the cell attachment on polystyrene surface. The cell growth curve of HGE which were cultured in KSFM with 50 g/L HSA was observed. Results The results showed significant decrease in cell numbers within 8 hours after HGE were inoculated,in which the polystyrene surface was preincubated with 50 g/L HSA. But it did not prove to be the case from 10 hours to 24 hours after HGE were inoculated.There were no significant difference within 24 hours in cell numbers between cultured in KSFM with 50 g/L HSA and control.The cell numbers in cell growth curve of HGE in KSFM with and without 50 g/L HSA did not show significant difference. Conclusions HSA preincubation on polystyrene were produce inhibitory effect of HGE attachment in early stage.

关 键 词：血清白蛋白 牙龈上皮细胞 细胞粘附 种植体 细胞培养 

分 类 号：R780.2[医药卫生—口腔医学]