大鼠延髓和脊髓背角的PKCγ阳性神经元向中脑导水管周围灰质的投射  

PKCγ-CONTAINING NEURONS IN THE MEDULLARY AND SPINAL DORSAL HORNS PROJECTING TO THE MIDBRAIN PERIAQUEDUCTAL GRAY IN THE RAT

作　　者：吴利平[1] 李辉[2] 李云庆[2] 张远强[3] 

机构地区：[1]第四军医大学西京医院惠宾病房,西安710032 [2]第四军医大学解剖学教研室暨梁琚脑研究中心,西安710032 [3]第四军医大学组织学胚胎学教研室,西安710032

出　　处：《解剖学报》2002年第1期6-10,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金 (3 9970 2 3 9;3 980 0 0 3 4;3 980 0 0 44 ) ;国家重点基础研究规划"脑功能和脑重大疾病的基础研究"(G19990 5 40 0 4) ;高等院校骨干教师资助计划资助项目

摘　　要：目的　观察延髓和脊髓背角内蛋白激酶Cγ亚单位 (PKCγ)样阳性神经元向中脑导水管周围灰质(PAG)的投射。　方法　荧光金 (FG)逆行追踪与PKCγ的免疫荧光组织化学染色相结合的双标记技术。　结果　PKCγ样阳性神经元主要分布于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核 ;将FG注入PAG后 ,在延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核内可见FG标记神经元 ;部分FG标记神经元呈PKCγ样阳性 ,FG PKCγ双标神经元也主要见于延髓和脊髓背角的Ⅰ、Ⅱ、Ⅲ层及脊髓的外侧脊核。　结论　延髓和脊髓背角的PKCγ阳性神经元可能参与将伤害性刺激信息向PAG的传递。Objective To examine the projection of protein kinase Cγ isoform(PKCγ)\|immunoreactive neurons from the medullary and spinal dorsal horns to the midbrain periaqueductal gray(PAG) in the rat. Methods By using fluoro\|gold(FG) retrograde tracing combined with immunofluorescence histochemical staining for PKCγ. Results PKCγ\|immunoreactive neurons were observed in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus.After injecting FG into the PAG,FG retrogradely labeled neurons were also mainly found in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus.Some of these FG\|labeled neurons in laminae Ⅰ,Ⅱ,Ⅲ of the medullary and spinal dorsal horns and lateral spinal nucleus exhibited PKCγ\|immunoreactivities.Conclusion\ PKCγ\|immunoreactive neurons in the medullary and spinal dorsal horns might be involved in the transmission of nociceptive information from the medullary and spinal dorsal horns to the PAG.\;[

关 键 词：蛋白激酶Cγ亚单位 延髓背角 脊髓背角 导水管周围灰质 伤害性感受 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]