人牙龈、牙周韧带细胞及牙槽骨细胞的体外培养研究Ⅰ.三种细胞生物学特性比较  

Culture of human gingival fibroblasts,periodontal ligament cells and alveolar bone cells Ⅰ.Biological characteristics comparision of the three types of cells

作　　者：葛少华[1] 杨丕山[1] 戚向敏[1] 孙钦峰[1] 

机构地区：[1]山东大学口腔医院

出　　处：《山东大学学报（医学版）》2002年第2期109-114,共6页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助项目 (Y98C0 80 37)

摘　　要：目的 :观察体外培养的牙周组织三种主要功能细胞的生物学特性差异。方法 :对人牙龈成纤维细胞 (GFs)、牙周韧带细胞 (PDLCs)及牙槽骨细胞 (ABCs)进行分离、培养、传代后 ,用MTT比色法、碱性磷酸酶测定法、考马斯亮蓝法及茜素红染色法测定其生物学活性。结果 :PDLCs和ABCs在碱性磷酸酶活性 (ALP)及矿化结节形成方面与GFs有显著性差异。结论 :尽管三种细胞具有相同的镜下表现 ,但生物学特性不同 ,则具有不同的表型 ,从而在牙周再生中发挥着不同的作用。Objective:To observe the different biological characteristics of the three types of cells which comprise the majority of cells in periodontium. Methods: Human gingival fibroblasts(GFs), periodontal ligament cells(PDLCs),alveolar bone cells(ABCs) were isolated,cultured and subcultured. MTT colorimetric assay, alkaline phosphatase activity(ALP)assay, Commasie brilliant blue staining and Dahl McGec Russel's alizarin red stain were used to determine their biological characteristics. Results: Compared with GFs, PDLCs and ABCs showed significant difference in ALP activity and mineralized nodule formation. Conclusion: Though GFs,PDLCs and ABCs had similar morphology under the microscope,they demonstrated different biological behavior in vitro in relation to their different phenotype , and therefore, they may play different roles in periodontal regeneration.

关 键 词：细胞培养 牙龈 牙周韧带 牙槽骨 

分 类 号：R781.4[医药卫生—口腔医学]