从人T细胞淋巴瘤cDNA文库中筛选含PH结构域编码序列的新基因  

Screening new genes containing PH domain coding sequence from human T cell lymphoma cDNA library

作　　者：李剑[1] 刘新平[1] 药立波[1] 林树新[2] 季少平[1] 聂晓燕[1] 

机构地区：[1]第四军医大学生物化学与分子生物学教研室,陕西西安710032 [2]第四军医大学病理生理学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2002年第1期27-29,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助;No.398707

摘　　要：目的从Ｔ细胞中获得更多含ＰＨ结构域编码序列的新基因。方法 采用低严谨核酸杂交技术，根据Ｔ细胞中已知的ＰＨ结构域的分子设计探针，对人Ｔ细胞淋巴瘤ｃＤＮＡ文库进行筛选。结果 在筛选过程中得到了４个新基因，各ｃＤＮＡ长度依次为：１７３３ｂｐ、２３０８ｂｐ、２１３０ｂｐ和１６４７ｂｐ，分别编码含２２９、３７６、２１１ 和 １４３个氨基酸残基的多肽。经生物信息学分析，在基因数据库中未发现与此４种ｃＤＮＡ序列类似的基因。该４个基因均已被ＧｅｎＢａｎｋ收录，登录号分别为：ＡＦ３３４５８８ＡＦ１３４５８９、ＡＦ３３４５９０和 ＡＦ３３４７９２。结论 这些新基因的获得及进一步研究，可能会为深人探讨Ｔ细胞激活机制提供新的线索。Aim To clone new genes containing PH domain coding sequence from human T cells. Methods A probe was designed ac- cording to the known molecules with PH domain in T cell ,then low stringency hybridization was used to screen human T cell lymphoma cDNA library. Results Four new genes were obtained. The cDNA length of then were 1 733 bp, 2 308 bp,2 130 bp,and 1 647 bp, en- coding 229,376,211 and 143 amino acids respectively. There were no similar sequences in the known gene databases. All these 4 genes were accepted by CenBank, with accession numbers AF334588, AF334589, AF334590 and AF334792. Conclusion Ob- taining of these new genes and further studying of them may provide new clues concering the Mechanisms of T cell aetivation

关 键 词：低严谨杂交 T细胞淋巴瘤 CDNA文库 筛选 PH结构域 

分 类 号：Q781[生物学—分子生物学] R733.4[医药卫生—肿瘤]