应用FISH技术对我国早期从事医用X线工作者的细胞遗传学评价  

CYTOGENETIC EVALUATION OF LONG-TERM IRRADIATION EFFECT ON WORKERS EARLY ENGAGED IN X-RAY MEDICINE IN CHINA BY FLUORESCENCE IN SITU HYBRIDIZATION

作　　者：孙元明[1] 李进[1] 王芹[1] 唐卫生[1] 王知权[1] 

机构地区：[1]中国医学科学院,中国协和医科大学放射医学研究所效应室,天津300192

出　　处：《癌变．畸变．突变》2002年第2期75-79,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：日本体质医学会基金;中国医学科学院中国协和医科大学基金 ( 95 2 0 6 0 )

摘　　要：目的 :对受到长期、低剂量率照射的我国早期从事医用X线工作者辐射损伤的远期效应进行细胞遗传学观察和评价。方法 :用 4号和 7号全染色体探针和荧光原位杂交技术 ,分析 2 5名早期X线工作者和 10名对照者外周血淋巴细胞染色体畸变 ,并用Giemsa染色验证。结果 :直观地表现出正常靶染色体和多种畸变。X线工作者的易位占其染色体总畸变的 84%;其他各种畸变率分别为插入 (Ins) 1.9%、双着丝粒 (Dic) 4 .5 %、无着丝粒断片 (Ace) 9.7%;X线工作者的染色体易位率和总畸变率均明显高于对照组 (P <0 .0 0 5 )。结论 :早期医用X线工作者外周血淋巴细胞的染色体畸变以易位为主。Purpose: To evaluate the cytogenetic effect of irradiation on the workers, early engaged in X-ray medicine in China, who had been exposed to X-ray at low dose rate for a long time (22-44 years). Method: The peripheral lymphocyte chromosome aberrations from 25 medical X-ray workers and 10 controls were analyzed by fluorescence in situ hybridization (FISH) with probes of chromosomes 4 and 7 and verified with Giemsa stai-{ning.} Results: The normal target chromosomes and their aberration were directly exhibited in morphology. The chromosomes translocations of X-ray workers were 84 % of all chromosome aberrations in this group. Ins (insertion), Dic (dicentric) and Ace (acentric fragment) were 1.9 %, 4.5 % and 9.7 %, respectively. The frequencies of chromosome translocation and total aberrations in X-ray workers were higher than that of the control group (P<0.005). Conclusion: The peripheral lymphocyte of medical X-ray workers exists chromosomes aberrations, the most part of which are translocations.

关 键 词：荧光原位杂交 X线工作者 染色体畸变 淋巴细胞 

分 类 号：R144.1[医药卫生—公共卫生与预防医学]