海湾扇贝幼虫变态过程中体内神经递质含量的变化  

THE CHANGES OF NEUROTRANSMITTER CONTENT DURING METAMORPHOSIS OF BAY SCALLOP ARGOPECTEN IRRADIANS LARVAE

作　　者：张涛[1] 阙华勇[1] 杨红生[1] 刘石林[1] 何义朝[1] 张福绥[1] 

机构地区：[1]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》2002年第3期239-244,共6页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目"双壳贝类幼虫变态机理研究"3 9970 5 88号;国家"973"课题资助项目 G1 9990 1 2 0 1 2号

摘　　要：采用生化方法测定了不同发育阶段及人工诱导后海湾扇贝 (Argopectenirradians)幼虫体内去甲肾上腺素 (NE)、多巴胺 (DA)和 5 羟色胺 ( 5 HT)含量的变化。结果表明 ,海湾扇贝幼虫体内去甲肾上腺素含量在变态前后没有明显变化 ,变态前为 2 35 2pg/mg湿重 ,变态后为2 770pg/mg湿重。多巴胺和 5 羟色胺含量在变态前 (第 1 3天 )急剧增加 ,比第 1 2天的幼虫分别增加了 2 .8倍和 4.7倍 ,变态后急剧下降 ,变态后幼苗比第 1 3天的幼虫分别降低了 2 5 .1倍和 1 6.4倍。海湾扇贝幼虫体内DA∶NE比值和 5 HT∶NE比值在变态前后变化剧烈。DA∶NE比值和 5 HT∶NE比值在变态前 (第 1 3天 )急剧增加 ,比第 1 2天的幼虫增加了 3.0倍 (DA∶NE)和 5 .0倍 ( 5 HT∶NE) ;变态后急剧降低 ,变态后幼苗比第 1 3天的幼虫降低了 2 9.8倍 (DA∶NE)和 1 9.5倍 ( 5 HT∶NE)。海湾扇贝幼虫经氯化钾和氯化钙诱导 2 4h后 ,体内去甲肾上腺素、多巴胺和 5 羟色胺以及DA∶NE比值和 5 HT∶NE比值均有所降低。本实验的结果表明 ,多巴胺和 5 羟色胺可能启动了海湾扇贝幼虫的变态过程。The changes of norepinephrine(NE),dopamine(DA)and 5 hydroxytryptamine(5 HT)in larvae of bay scallop Argopecten irradians at different development stages and after induced by KCl and CaCl 2 have been studied.The results show that NE levels in the corresponding larva and spat groups do not change significantly,with 2352(pg/mg WW)(before metamorphosis,13d)and 2770(pg/mg WW)(spats ). DA and 5 HT levels are low in young larvae,then increase with larvae developing.DA and 5 HT levels increase drastically just before metamorphosis(13d),which increase 2.8 and 4.7 times respectively compared to 12 day larvae.After metamorphosis,DA and 5 HT levels decrease drastically,which in spats following metamorphosis decrease 25.1 and 16.4 times respectively compared to 13 day larvae.The DA∶NE ratio and 5 HT∶NE ratio also change drastically among 12 day larvae,13 day larvae and spats.The DA∶NE ratio and 5 HT∶NE ratio increase drastically just before metamorphosis,which increase 3.0 and 5.0 times,respectively,and then decrease drastically after metamorphosis.The DA∶NE ratio and 5 HT∶NE ratio in spats decrease 29.8 and 19.5 times compared to 13 day larvae.The NE,DA and 5 HT levels and DA∶NE ratio,5 HT∶NE ratio decrease after induced by KCl and CaCl 2.The results suggest that DA and 5 HT maybe trigger the metamorphosis process of bay scallop A.irradians.

关 键 词：海湾扇贝 去甲肾上腺素 多巴胺 5-羟色胺 变态 神经递质 

分 类 号：S968.313[农业科学—水产养殖]