VA菌根对土壤中DEHP降解的影响  

Effect of VA mycorrhiza on degradation of DEHP in soil

作　　者：王曙光[1] 林先贵[1] 尹睿[1] 

机构地区：[1]中国科学院南京土壤研究所,南京210008

出　　处：《环境科学学报》2002年第3期369-373,共5页Acta Scientiae Circumstantiae

基　　金："973"国家重点基础研究和规划项目 (G19990 1180 7)

摘　　要：以豇豆为供试植物 ,分别接种泡囊丛枝菌根 (VAM)真菌Acaulosporalavis(光壁无梗球囊霉 ,菌号 :34)和Glomuscaledoni um(苏格兰球囊霉 ,菌号 :90 0 36 ) ,研究VA菌根对菌根际 (A)、菌丝际 (B)和常规土 (C)土层中不同浓度DEHP(4、2 0、10 0mg kg)降解的影响 .试验持续 6 0d .结果表明 :接种VAM真菌促进了DEHP在A、B、C土层中的降解 ,尤其在B层的降解 ,说明菌丝在DEHP降解和转移过程中起了重要作用 .其中 34号菌接种效果较为显著 ,A、B土层中的DEHP残留浓度分别比不接种最大下降 2 5 1%和 10 1% .受VAM直接影响的A、B土层中细菌、真菌、放线菌数量呈下降趋势 ,而在C层中呈增加趋势 ,A、B、C土层中土壤中性磷酸酶活性也呈现出同样变化趋势 .Pigna sinensis was inoculated with vesicular arbuscular mycorrhiza(VAM) fungi Acaulospora lavis (strain No.34) and Glomus caledonium (strain No.90036) respectively to study the effects of VAM on degradation of DEHP at different concentrations (4、20、100?mg/kg) in mycorrhizosphere(A), hyphosphere(B) and formal soil(C). The expeiment lasted 60 days. The results showed that VAM fungi could improve the degradation of DEHP in A, B and C soils layers, especially in B layer, which indicated that hyphae played an important role in degradation and translocation of DEHP. The inoculating effect of strain No.34 was more obvious,compaved with the uninoculated and the residues of DEHP in A and B decreased by 25 1% and 10 1% respectively. The numbers of bacteria, fungi and actinomycetes decreased in A and B layers which were directly affected by VAM, but increased in C layer. The activity of soil neutral phosphatase in A, B and C layer soils changed in a same way as the number of soil microorganisms. The decrease of number of soil microorganisms and activity of soil neutral phosphatase in A and B may negatively affect the role of VA mycorrhiza played in degrading DEHP.

关 键 词：VA菌根 土壤 VAM 接种 残留浓度 降解 DEHP 土壤污染 

分 类 号：X172[环境科学与工程—环境科学] X53