舌癌颈淋巴结微转移灶的免疫组化检测  

Immunohistochemical Detection of Micrometastases in Cervical Lymph Nodes from Squamous Cell Carcinoma of Tongue in Neck Dissection Specimens

作　　者：李伟忠[1] 赵彤[1] 张进华[1] 殷学民[1] 薛宏宇[1] 王天舒[1] 

机构地区：[1]第一军医大学附属南方医院

出　　处：《华西口腔医学杂志》2002年第3期181-183,共3页West China Journal of Stomatology

摘　　要：目的 :探讨免疫组织化学S_P微波法对阴性淋巴结微转移灶的检出率。方法 :采用S_P微波法 ,对 30例舌鳞状细胞癌患者既往报告为阴性的 15 2个淋巴结的石蜡标本再作切片 ,并作免疫组织化学染色。取阳性淋巴结作为对照。结果 :30例患者 15 2个阴性淋巴结 ,经CK(AE1/AE3)单克隆抗体标记后 ,在 4例 (13 3% )患者中共 7个淋巴结 (4 6 % )内CK(AE1/AE3)单克隆抗体呈阳性反应。癌细胞呈单个或呈簇状散在于淋巴结内 ,淋巴结结构完好。检出的 4例患者 ,高分化及低分化鳞状细胞癌各 2例 ,其中 2例术前已发现颈部有肿大淋巴结 ,7个微转移之淋巴结中 ,颌下区 2 ,颈深上 4 ,颈深中 1。正常淋巴结呈阴性反应。结论 :舌癌患者的阴性淋巴结中 ,仍有一定数量的微转移灶发生 ,采用免疫组化的方法 。Objective: The purpose of this study was to explore a feasible method to detect the micrometastases.Methods: Totally 152 cases of negative cervical lymph nodes (CLNs) from 30 patients with squamous cell carcinoma in tongue were included in this study. The HE_stained slices of the CLN were reexamined by two experienced pathologists and, conformed that no carcinoma cells were found. Two slices were made from each paraffin specimen and, the slices were stained with the microwave immunohistochemical technique with monoclonal antibody CK (AE1/AE3) (DAKO Co. Denmark, 1∶100).Results: Among these 152 cases 7 (4 6%) positive lymph nodes were found in 4(13 3%) patients, and CLN metastases were found in all the patients before the surgical treatment. Most of the micro_metastatic nodes appeared in the upper deep cervical area, except that one of them was found in the submandibular triangle.Conclusion: The results suggest that micrometastases frequently occurred in negative lymph nodes. The present method may be useful in detecting the micrometastases of lymph nodes and in evaluating clinical stages of patients with oral cancers.\;

关 键 词：免疫组化 检测 舌鳞状细胞癌 微转移灶 淋巴结 

分 类 号：R739.8[医药卫生—肿瘤]