恶性成釉细胞瘤定量病理学研究  

A Quantitatively Pathological Study on Malignant Ameloblastomas

作　　者：刘进忠[1] 汪说之[1] 陈新明[1] 

机构地区：[1]武汉大学口腔医学院

出　　处：《华西口腔医学杂志》2002年第3期187-189,共3页West China Journal of Stomatology

摘　　要：目的 :探讨恶性成釉细胞瘤细胞核形态参数、DNA含量及AgNOR形态分型和计数在诊断中的意义。方法 :选取 17例恶性成釉细胞瘤患者的切除标本行HE、AgNOR及DNA染色 ,应用图像分析仪对细胞核形态几何参数、DNA含量及倍体、AgNOR进行定量分析。结果 :在细胞核的几何参数中 ,恶性成釉细胞瘤细胞的面积、周长、等圆直径、短径、平均直径、圆度、轴比与良性成釉细胞瘤均有显著性差异 (P <0 0 1)。恶性成釉细胞瘤AgNOR计数和DNA指数显著高于良性成釉细胞瘤 (P <0 0 1)。根据核形态参数和DNA指数建立了判别恶性成釉细胞瘤的回归方程。结论Objective: The purpose of this study was to investigate the role of nuclear morphometric parameters, DNA content and AgNOR count in the differentiating malignant and benign ameloblastomas.Methods: Totally 17 cases of malignant ameloblastomas were examined by using HE, AgNOR and DNA stain methods. Morphometric parameters of cell nuclei, DNA content and AgNOR count were quantitatively studied by using an image analysis system.Results: Seven parameters (area, perimeter, equal diameter, minor diameter, mean diameter, round index, axis ratio) out of ten shape factors were significantly different between malignant and benign ameloblastoma (P<0 01). AgNOR count and DNA index in malignant ameloblastoma were significantly higher than those in benign ameloblastoma (P<0 01). Logistic regression equation was established, according to nuclear morphormetric parameters and DNA index.Conclusion: Quantitative analysis of DAN content, nuclear morphmetric parameters and AgNOR count may be helpful in differentiating malignant and benign ameloblastomas.\;

关 键 词：成釉细胞瘤 定量病理学 形态计量 DNA含量 核仁组成区 图像分析 

分 类 号：R739.8[医药卫生—肿瘤]