黄孢原毛平革菌合成漆酶能力的研究  

THE STUDY OF LACCASE PRODUCTION CONDITIONS OF PHANEROCHAETE CHRYSOSPORIUM

作　　者：段新源[1] 卢雪梅[1] 王蔚[1] 高培基[1] 

机构地区：[1]山东大学微生物技术国家重点实验室,济南250100

出　　处：《山东大学学报（理学版）》2002年第1期91-94,共4页Journal of Shandong University(Natural Science)

基　　金：国家自然科学基金 ( 2 990 6 0 0 5 )资助项目

摘　　要：全面的研究了各种影响漆酶合成的条件对漆酶的产生的影响 .P .chrysosporium在静置条件下 ,无论充氧与否 ,均不产生漆酶 .在固体与半固体条件下 ,也不产生漆酶 ,仅在 :高氮 (2 4mmol/L) ,高铜 (16 0 μmol/L) ,摇床 (118r/min) ,30℃ ,6d可产少量漆酶 .在P .chrysosporium对木素的降解中 。The factors that can effect the production of laccase were studied in this work.P.chrysosporium can not product obviouly laccase in static conditions whether flushed with 100% oxygen or not.It also does not produce laccase in solid and semi-solid conditions.Low level of laccase were detected with shaking(118 r/min),30 ℃,6 days.Laccase production was not efficiently increased under all the conditions studied.With such a low activity,laccase can not play an important role in delignification by P.chrysosporium.

关 键 词：合成 黄孢原毛平革菌 漆酶 木素 生物降解 培养条件 产酶能力 碳源 

分 类 号：TQ925.9[轻工技术与工程—发酵工程]