隐孔菌多糖对肺组织释放白三烯的影响  

Effects of polysaccharides from cryptoporus volvatus(peck) schear on leukotrienes generation in guinea pig lung

作　　者：周建仓[1] 谢强敏[1] 季华[1] 金赛红[1] 陈季强[1] 

机构地区：[1]浙江大学医学院国家药品监督管理局浙江呼吸药物研究实验室,杭州310031

出　　处：《中国药理学通报》2002年第2期169-172,共4页Chinese Pharmacological Bulletin

摘　　要：目的　观察发酵隐孔菌多糖 (以下称隐孔菌多糖 )对豚鼠肺组织释放白三烯及气管Schultz Dale反应的影响。方法　采用生物检定法和反相高效液相色谱法 (RP HPLC)测定致敏豚鼠肺组织抗原攻击释放过敏性慢反应物质 (SRS A)和正常豚鼠肺组织A2 3187攻击释放白三烯D4(LTD4) ,以及用致敏豚鼠气管Schultz Dale法检测隐孔菌多糖的抗变态反应活性。结果　隐孔菌多糖明显减少抗原攻击致敏豚鼠肺组织SRS A释放量和A2 3 187攻击正常豚鼠肺组织LTD4释放量 ;抑制致敏豚鼠气管Schultz Dale反应 ,IC50 =0 4 9g·L-1。AIM To study the effects of polysaccharides of ferment cultured cryptoporus volvatus (CVPS) on release of leukotrienes from guinea pig lung and Schultz Dale reaction. METHODS Thebioassay method and reversed phase high performance liquid chromatography (RP HPLC) were used to analyze SRS A or LTD 4 from sensitized or normal guinea pig, after challenged with antigen or A 23187 respectively. The antiallergic effects of CVPS were evaluated with Schultz Dale reaction. RESULTS The release of SRS A or LTD 4 from sensitized or normal guinea pigs were greatly reduced by CVPS after antigen or A 23187 challenge. The CVPS could also inhibit the Schultz Dale reaction of sensitized guinea pig (IC 50 =0 49 g·L -1 ). CONCLUSION The effects of CVPS involved in inhibiting release of leukotrienes from lung and antiallergy.

关 键 词：隐孔菌 多糖 过敏性慢反应物质(SRS-A) 白三烯D4(LTD4) 反相高效液相色谱(RP-HPLC) 肺 豚鼠 

分 类 号：R－332[医药卫生] R284.1