二烯丙基三硫对胃癌MGC-803细胞的生长抑制作用  

Effect of growth inhibition of diallyl trisulfide on gastric cancer MGC-803 cell line in vitro

作　　者：彭军[1] 苏琦[1] 宋颖[1] 张良运[1] 梁晓秋[1] 

机构地区：[1]南华大学肿瘤研究所

出　　处：《中国药理学通报》2002年第2期210-213,共4页Chinese Pharmacological Bulletin

基　　金：湖南省教委科研基金资助课题No 99c2 0 3

摘　　要：目的　研究二烯丙基三硫 (DATS)在体外对胃癌MGC 80 3细胞的生长抑制作用。方法　采用MTT法、集落形成率及生长曲线绘制分别观察不同浓度DATS对胃癌MGC 80 3细胞生长的影响。结果　DATS处理后 ,培养的MGC 80 3细胞增殖抑制而稀少。不同浓度DATS对胃癌MGC 80 3细胞的作用 ,4、8、12、16、2 4mg·L-1的细胞生长抑制率分别为 2 6 %、4 6 %、6 5 %、76 %、89% ,其半数抑制浓度(IC50 )为 8 2mg·L-1。 8、12、16、2 4mg·L-1的细胞集落形成率和集落形成相对数各为 32 4 %和 5 8 7%、2 4 8%和4 2 5 %、19%和 33 5 %、8 8%和 15 1% ,皆具有明显的量效关系 ,且随着浓度增高 ,细胞生长曲线趋于低平。结论　DATS在体外对胃癌MGC 80AIM To study the effects of growth inhibition of different concentrations of diallyl trisulfide(DATS) on gastric cancer MGC 803 cell line in vitro. METHODS The influence of different concentrations of DATS were examined by MTT assay, clonal formation rates and cell growth curve. RESULTS Suppression and decrease of MGC 803 cell proliferation was found after treatment by DATS in vitro. The inhibitory rates on MGC 803 cell growth of different concentration of DATS,4, 8, 12, 16 and 24 mg·L -1 , were 26%,46%,65%,76% and 89% respectively, and its half inhibitory concentration (IC 50 ) was 8 2 mg·L -1 . The clonal formation rates and clonal formation relative counts of 8, 12, 16 and 24 mg·L -1 were 32 4% and 58 7%,24 8% and 42 5%,19 0% and 33 5%?8 8% and 15 1% respectively.There was significant correlation between dose and effect in all, and the cell growth culve became lower and flatter when concentration of DATS increase gradually. CONCLUSION The effect of growth inhibition of DATS for gastric cancer MGC 803 cell in vitro is remarkable.

关 键 词：胃肿瘤 MGC-803细胞 二烯丙基三硫 生长抑制 

分 类 号：R735.2[医药卫生—肿瘤]