免疫荧光法检测L-NNA对脑缺血再灌流后海马区GFAP合成的影响  

Effect of L-NNA on GFAP production in the gerbil hippocampus after recirculation following ischemia

作　　者：张敬军[1] 陈青[2] 夏作理[1] 

机构地区：[1]泰山医学院附属医院神经内科,泰安271000 [2]山东泰安卫生学校,泰安271000

出　　处：《中国药理学通报》2002年第2期217-219,共3页Chinese Pharmacological Bulletin

基　　金：山东省自然科学基金资助课题NoY2 0 0 0C2 3

摘　　要：目的　探讨NG 硝基 L 精氨酸 (L NNA)对沙土鼠海马区胶质纤维酸性蛋白 (GFAP)合成的影响。方法　钳夹沙土鼠的双侧颈总动脉制造脑缺血模型 ,应用免疫荧光法染色。结果　脑缺血再灌流后海马区GFAP合成增加 ,GFAP阳性细胞主要分布在放射层及分子层 ,L NNA能抑制海马区GFAP的合成。结论　L NNA是一氧化氮合酶强的抑制剂 ,LAIM To observe the effects of L NNA on glial fibrillary acidic protein (GFAP) production in the gerbil hippocampus after recirculation following ischemia. METHODS Both vessels occlusion and immunofluorescent methods in gerbil hippocampal tissue slices incubated in vitro were used. RESULTS Recirculation following ischemia led to a rise on GFAP concentration in the hippocampus. GFAP positive cells mainly distributed in rediatum layer and molecullar layer in the hippocampus, L NNA decreased GFAP synthesis. CONCLUSION L NNA is a strong inhibitor of nitric oxide synthase(NOS). It is possible that the role of endogenous NO mediated GFAP synthesis is inhibited by L NNA.

关 键 词：L-NNA 脑缺血 海马 胶质纤维酸性蛋白 

分 类 号：R－332[医药卫生] R743.31