表达屋尘螨Ⅰ类变应原Der p1的基因工程菌的发酵条件探讨  

Studies on Fermentation Conditions of GeneticallyEngineered Escherichial Coli Expressing the MajorHouse Dust Mite Allergen Der p 1

作　　者：刘向辉[1] 卢文菊[1] 都昌胡[2] 徐军[2] 

机构地区：[1]广州医学院检验系,广州510182 [2]广州市呼吸疾病研究所,广州510120

出　　处：《广州医学院学报》2002年第1期46-47,52,共3页Academic Journal of Guangzhou Medical College

摘　　要：目的：探讨发酵条件对大肠杆菌工程菌表达重组屋尘螨I类变应原（Der p1)的影响。方法：应用摇瓶发酵试验观察不同诱导时间和不同诱导时机对Der p1表达量的影响。结果：（1）Der p 1基因在E.coliBL21（pBVDP8)菌密度A600nm0.6左右时，42℃最佳诱导时间为4h.(2)工程菌E.coli BL21(pBVDP8)在30℃培养至A600nm为0．5－0．7时，42℃诱导4hDerp 1表达量最高，结论：确定了最适诱导时间和诱导时机，初步形成了一套高表达Der p 1的发酵技术。Objective: To investigate the effects of fermentation conditions on the production of the major house dust mite allergen, Dermatophagoides pteronyssinus 1 ( Der p 1) by genetically engineered E. coli. Methods: The effects of different cell growth phases for induction and different inducing period on Der p 1 production were observed using shaking flask and fermentor respectively. Results; (1) when the cultivation of E. coli BL21 ( pBVDPS) in shaking falsk reached to A600nm 0. 6 at 42 ℃ , the best inducing period was 4 hours. (2) when the cultivation of E. coli BL21 ( pBVDPS) in shaking flask reached to A600nm0. 5 - 0. 7 at 30℃ and induced at 42 ℃ for 4 hours , the yield of Der p 1 reached to the highest level. Conclusion; The optimal growth phase for induction and the inducing period were determined.

关 键 词：屋尘螨I类变应原 基因表达 大肠杆菌 发酵 基因工程菌 Der P1 

分 类 号：Q78[生物学—分子生物学]