L型细菌感染对胆囊黏膜肿瘤坏死因子和血小板源生长因子水平的影响  

Influence of L-form infection on tumor necrosis factor and platelet-derived growth factor levels in gallbladder mucosa

作　　者：焦兴元[1] 黄洁夫[1] 吕明德[1] 梁力健[1] 

机构地区：[1]中山医科大学附属第一医院肝胆外科,广东广州510080

出　　处：《中国普通外科杂志》2002年第2期102-105,共4页China Journal of General Surgery

摘　　要：目的　 探讨肿瘤坏死因子 α(TNF -α)和血小板源生长因子 (PDGF )在L型细菌感染相关性胆囊癌发病中的变化。 方法　 采用RIA法测定胆囊癌、胆囊炎病人和对照组病人胆囊黏膜培养上清液中TNF -a含量 ,采用细胞生物活性法测定PDGF活性。 结果　 胆囊癌、胆囊炎和正常胆囊的胆囊黏膜L型细菌检出率分别为 80 .0 % ( 16 / 2 0 ) ,82 .5 % ( 33/ 40 )和 0 ( 0 / 2 0 )。L型阳性胆囊癌胆囊黏膜TNF -a和PDGF活性均明显高于L型阴性胆囊癌、L型阳性胆囊炎和对照组。 结论　 L型细菌在胆囊癌的发病过程中 ,可能首先引起胆囊黏膜表达PDGF 。Objective To investigate the changes of tumor necrosis factor-α(TNF-α) and platelet-derived growth factor(PDGF) in gallbladder carcinoma (GA) patients with cell wall-deficient bacleria(L-form) infection .Methods The levels of TNF-α in gallbladder mucosa were detected by radioimmunoassay and the activity of PDGF was detected by bioactivity assay in patients with GA or cholecystitis and normal gallbladder(control group).Results The positive detection rates of L-forms in gallbladder mucosa of gallbladder carcinoma , chronic cholecystitis, and control group were 80.0%(16/20),82.5%(33/40) and 0(0/20), respectively.The TNF-α and PDGF levels in patients with L-form infection-positive gallbladder carcinoma were significantly higher than those in patients without L-form infection and in control group.The levels of TNF-a and PDGF in patients with L-form infection positive chronic cholecystitis were significantly higher than those in patients without L-form infection and in control group.Conclusions L-form may be one of the direct factors leading to the increase in PDGF during gallbladder oncogenesis,and there is positive correlation between PDGF and gallbladder oncogenesis when L-form induces inflammatory reaction and predisposes gallbladder mucosa to develope neoplasms.

关 键 词：肿瘤坏死因子 血小板源生长因子 血液 胆囊肿瘤 L型菌 病因学 

分 类 号：R735.8[医药卫生—肿瘤] Q592.1[医药卫生—临床医学]