角蛋白阳性细胞检测在肿瘤转移诊断中的意义  

Significance of detection of cytokeratin positive cells in bone marrow in the diagnosis of cancer metastasis

作　　者：李洁[1] 周童亮[1] 赵武述[1] 刘德若[2] 赵洪昌[2] 陈应华[3] 

机构地区：[1]中日友好临床医学研究所免疫室,北京100029 [2]中日友好医院胸外科,北京100029 [3]清华大学生命科学与工程研究院,北京100083

出　　处：《中日友好医院学报》2001年第6期323-326,共4页Journal of China-Japan Friendship Hospital

基　　金：中日友好医院 -清华大学基金

摘　　要：目的 :探讨建立早期诊断肿瘤转移的方法。方法 :用免疫组化方法检测癌症患者的骨髓涂片中的角蛋白阳性细胞。结果 :2 1株人的癌细胞株中 ,凡上皮来源者均呈阳性反应 ,非上皮来源者均呈阴性反应。 2 4例癌症患者中 ,18例骨髓中有角蛋白阳性细胞 ,其中 1/ 3的患者淋巴结没有转移。另外冰冻检查发现 ,分化不明显的癌细胞角蛋白表达更多 ,染色更深。结论 :骨髓中角蛋白阳性细胞检测技术 ,可用于某些癌症血行转移的早期诊断和形态分化不明确。Objective:To study the method of early diagnosis of cancer metastasis.Methods:Detection of cytokeratin positive cells in the bone marrow with immunohistochemical method. Results:21 human tumor cell lines and bone marrow from 24 cancer patients were detected.All of the examined tumor cell lines originated from epithelium were keratin positive,while the non epithelial tumor cell lines were negative .keratin positive cells were found in 18 of 22 bone marrows from patients with epithelium derived cancer,and 1/3 of them without any metastasis in their lymph nodes.In addition, examinations with ice section of tumor tissues displayed that undifferentiated tumor cells expressed more intensive keratin molecules.Conclusion:The method to detect keratin positive cells in bone marrow could be used to early diagnose blood cir culatiag cancer metastasis and to be used as a complementary discrimination diagnosis.

关 键 词：骨髓涂片 免疫组化 角蛋白阳性细胞 检测 肿瘤转移 

分 类 号：R730.43[医药卫生—肿瘤]