P73基因在长春新碱诱导髓性白血病细胞系U937细胞凋亡过程中的表达  

P73 GENE EXPRESSION IN THE PROCESS OF ACUTE MYELOID LEUKEMIA CELL LINE U937 APOPTOSIS INDUCED BY VINCRISTINE SULFATE

作　　者：肖广芬[1] 卢青[2] 杨向东[3] 

机构地区：[1]南华大学附二医院血液内科,衡阳421001 [2]浙江省杭州市第一人民医院,310006 [3]南华大学心血管病研究所

出　　处：《中国现代医学杂志》2002年第5期31-34,共4页China Journal of Modern Medicine

摘　　要：目的 :探讨长春新碱诱导U937细胞凋亡过程中P73mRNA的表达变化 ,以进一步了解P73基因在U937细胞凋亡中的作用。方法 :用长春新碱诱导U937细胞凋亡 ,凋亡指标采用细胞形态学、DNA片段电泳、流式细胞术检测DNA含量等方法 ;采用半定量逆转录PCR(RT -PCR)检测P73mRNA的表达变化。结果 :长春新碱可诱导U937细胞凋亡。 2 0 μg/ml的长春新碱使用于U937细胞 1 8h ,光镜下见细胞明显聚集、体积缩小 ,荧光染色见染色质明显浓缩、核碎裂等现象 ,而对照组未出现上述现象 ;DNA片段电泳见清晰的梯形条带 ;流式细胞仪检测 :长春新碱作用于U937细胞 1 8h ,2 4h ,细胞的凋亡率分别为 1 0 .54 %、35 .1 5 % ,而对照组的凋亡率仅为 0 .93 % ;半定量RT -PCR结果 :在U937细胞凋亡前后 ,P73mRNA的表达差异无显著性。结论Objective:To study the variation of p73 gene expression in the process of acute myeloid leukemia (AML) cell line U937 apoptosis induced by Vincristine sulfate (VCR). Methods:Morphological changes of apoptosis were observed with invert microscope and fluorescence staining.DNA Ladder and Sub-G1 Peak were examined by DNA gel electronphores and fluorescence flow cytometry (FCM), respectively.Using semi-quantitive reverse transcription-polymerase chain reaction (RT-PCR) to examine the expression of p73 mRNA.Results:VCR can induce U937 cell apoptosis effectively .Condense of nuclear,fragmentations of chromosome and DNA Ladder were seen after 18h following treatment of 20mg/L VCR.After being treated with 20mg/L VCR for 18h and 24h,the rate of apoptosis determined by FCM in U937 cell was 10.54%,35.15% respectively.But the quantity of p73 expression was stable.Conclusion:P73 mRNA level was not changed in the process of U937 cell's apoptosis induced by VCR.

关 键 词：P73 细胞凋亡 U937细胞 髓性白血病 

分 类 号：R733.7[医药卫生—肿瘤]