F VⅢ基因的分子遗传学研究  

A study of factor Ⅷ gene with molecular genetics.

作　　者：朱霖[1] 杨善志[1] 杨辉[1] 谢向农[1] 张旭[1] 叶文虎[1] 

机构地区：[1]安徽省优生优育遗传医学中心,230031

出　　处：《中国优生与遗传杂志》2001年第4期13-14,12,共3页Chinese Journal of Birth Health & Heredity

摘　　要：对若干个不相关个体的FVⅢ基因进行了 4个基因内的RFLPs(BclI、HindⅢ、XbalⅠ、MspⅠ )及几个基因外的RFLPs(TaqⅠ、BstxⅠ、PstⅠ、AccⅠ )的研究 ,计算了FVⅢ基因内多态位点的杂合子频率 ,在内含子 2 2处 ,发现了两个额外的XbalⅠ多态位点。在FVⅢ基因外的DXS5 2区域 ,用探针St14- 1检测到两个TaqⅠ多态系统 ,并计算了杂合子频率。同时观察到在BclⅠ、XbalⅠ。Four intragenic RFLPs(Bcl I,Hind Ⅲ,Xbal I,Msp I)and several extragenic RFLPs(Taq I,Bstx I,Pst I,Acc I)of the factor Ⅷ gene were studied in a number of unrelated population.The frequencies of heterozygous with factor Ⅷ gene were calculated .Two additional Xbal I polymorphisms in intron 22 were observed.With probe St14-1 and restriction enzyme Taq I,two systems were tested in the extragenic DXS52.Linkage disequilibrum to some degree among Bcl I,Xbal I,Msp I were observed.

关 键 词：甲型血友病 限制性片段长度多态性 凝血因子Ⅷ FVⅢ基因 分子遗传学 研究 

分 类 号：R346[医药卫生—基础医学]