食管癌Fas和bcl-2蛋白和DNA含量的定量研究  

Quantitative study on the Fas and bc1-2 protein expression and DNA content in esophageal carcinoma

作　　者：刘丽萍[1] 涂响安[1] 赵志毅[1] 张明[1] 左连富[2] 

机构地区：[1]广东省肇庆市第一人民医院,广东肇庆526021 [2]河北省肿瘤研究所,河北石家庄050011

出　　处：《右江民族医学院学报》2002年第2期186-188,共3页Journal of Youjiang Medical University for Nationalities

摘　　要：目的　探讨Fas和bcl- 2蛋白表达和DNA倍体异常在食管癌发生和发展中的作用。方法　应用流式细胞术和细胞免疫荧光技术对 70例患者食管鳞癌组织Fas和bcl- 2蛋白和DNA含量进行定量检测。结果　异倍体 36例 (51 .4 % ) ,二倍体 34例 (48.6 % ) ;异倍体肿瘤其DNA指数、增殖指数和S期细胞比率均高于二倍体肿瘤 ,但差异无显著性 (P>0 .0 5) ;Fas蛋白阳性表达 42例 (60 .0 % ) ,bcl- 2蛋白阳性表达 65例 (92 .9% ) ,Fas蛋白表达与肿瘤病理分级密切相关(P <0 .0 5) ;异倍体肿瘤和二倍体肿瘤Fas和bcl- 2阳性表达率和表达量差异均无显著性 (P >0 .0 5)。结论　DNA倍体异常、Fas和bcl- 2蛋白的异常表达在食管癌发生和发展中起重要作用。Objective To investigate the roles of Fas and bc1-2 protein expression and abnormal DNA ploidy in the occurrence and development of esophageal carcinoma. Methods A flow cytometry and a cellular immunofluoresence technique were used to quantitatively measure the Fas and bc1-2 protein and DNA content in tissues from 70 patients with esophageal carcinoma, respectively. Results 36 cases were found out with abnormal ploidy (51.4%), and 34 with diploidy (48.6%); all the DNA and proliferation indexes and stage Scellular radio in abnormal ploidy tumor were higher than those in diploidy tumor, but there were no significant differences between them (P>0.05); 42 cases with positive Fas protein expression (60.0%), 65 with positive bc1-2 protein (92.9%), the Fas protein expression had closely correlation with tumor pathology stage (P<0.05); there were no significant difference between positive expressive rate and expression content in Fas and bc1-2 in abnormal ploidy and diploidy tumors (P>0.05). Conclusions The abnormal DNA ploidy, Fas and bc1-2 protein expression play an important role in the occurrence and development of esophageal carcinoma.

关 键 词：食管肿瘤 基因 蛋白 DNA 流式细胞术 荧光抗体技术 

分 类 号：R735.1[医药卫生—肿瘤]