脐血造血干/祖细胞端粒酶相关蛋白基因hTERT和TEP1的表达及意义  

The expression of human telomerase-associated protein hTERT and TEP1 in cord blood stem/progenitor cells and its significance

作　　者：马艳萍[1] 邹萍[1] 肖娟[1] 黄士昂[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,武汉430022 [2]美国加洲大学

出　　处：《中华血液学杂志》2002年第4期183-186,共4页Chinese Journal of Hematology

基　　金：国家自然基金海外青年学者合作研究基金资助项目( 3992 80 10 )

摘　　要：目的　探讨造血干 祖细胞端粒酶相关蛋白基因hTERT和TEP1在造血过程中对端粒酶活性的调节作用。方法　用RT PCR和TRAP法分别测定脐血干 祖细胞中hTERT、TEP1mRNA及端粒酶的活性。结果　在新分离的脐血单个核细胞 (MNC)和CD3 4 - 细胞中检测不到端粒酶的活性 ,hTERTmRNA表达阴性 ;CD3 4+细胞中端粒酶活性低 ,hTERTmRNA阳性 ;而TEP1mRNA在MNC、CD3 4- 细胞和CD3 4+细胞中均为阳性 ,表达水平差异无显著性。在不同细胞因子组合条件下 ,CD3 4+细胞体外培养 7d ,细胞端粒酶活性和hTERTmRNA表达增高 ,之后随着细胞的分化成熟 ,二者表达降低 ,在整个培养过程中 ,TEP1mRNA表达无明显变化。结论　在脐血造血干 祖细胞中 ,hTERT基因表达水平与端粒酶活性一致 ,hTERT基因表达水平对端粒酶的活性起关键作用 。Objective To explore the regulatory effects of hTERT and TEP1 on telomerase activity in hematopoiesis. Methods The hTERT and TEP1 mRNA expression was detected by RT-PCR and the telomerase activi-ty by TRAP. Results In mononuclear cells (MNC) and CD 34 - cells, no detectable telomerase activity and hTERT mRNA expression were found. CD 34 + cells showed hTERT expression and a low level telomerase activity.TEP1 mRNA was detected in MNC, CD 34 - and CD 34 + cells with no significant difference in the expression level. In the CD 34 + cells cultured in vitro with growth factors for 7 days, the telomerase activity and the expression of hTERT mRNA were upregulated, but were downregulated in the long time culture. No significant changes in TEP1 expression was observed. Conclusion In the course of hematopoiesis, hTERT mRNA expression was in accordance with telomerase activity, hTERT gene plays a crucial role in the expression of telomerase activity, while TEP1 gene plays, if any, a much smaller role.

关 键 词：端粒酶 HTERT基因 TEP1基因 造血干细胞 基因表达 肿瘤标志物 

分 类 号：R730.2[医药卫生—肿瘤]