热休克对急性运动大鼠骨骼肌中MDA及SOD的影响  

The Change of MDA and SOD in Skeletal Muscle with Single-bout Exercise after Heat Stress in Rat

作　　者：叶春[1] 王瑞元[2] 丁益[3] 何执静[3] 李建平[4] 

机构地区：[1]南京师范大学体育科学学院,南京210097 [2]北京体育大学 [3]南京大学生物医药技术国家重点实验室 [4]中央民族大学体育系

出　　处：《中国运动医学杂志》2002年第3期253-255,共3页Chinese Journal of Sports Medicine

摘　　要：目的 :探讨热休克对急性运动后大鼠骨骼肌中脂质过氧化物 (MDA)和抗氧化酶 (SOD)的影响。方法 :SD大鼠分为高温安静组、高温运动组、室温安静组、室温运动组。高温安静组和高温运动组大鼠经热休克处理后室温恢复 2 4小时 ,进行一次性急性运动 ,运动后 16小时取样进行各项检测并与室温安静组和室温运动组进行比较。结果发现 ,高温安静组HSP72高于室温安静组 ,高温运动组骨骼肌中MDA明显低于室温运动组 (P <0 0 5 ) ,而SOD略高于室温运动组 ,但无统计学意义 (P >0 0 5 )。结果提示 ,热休克可明显减少骨骼肌中脂质过氧化物 (MDA)含量 。This study tested the hypothesis that heat stress respond is associated with the change in rat skeletal muscle with single-bout exercise. Male rats were assigned to one of the following four experimental groups (n = 10/group): (1) normothermic sedentary (Norm Sed), (2) heat stress (Heat Sed), (3) normothermic exercise (Norm Exe), or (4) heat stress before exercise (Heat Exe). Animals in the Heat Sed and Heat Exe groups were exposed to 15 min of whole body hyperthermia (colonic temperature 41℃～41.5℃). Twenty-four hours after heat stress respond, both the Norm Exe and Heat Exe groups were subjected to single-bout exercise. After exercise, the animals were anesthetized, and the gastrocnemius were removed, and SOD, MDA and the relative levels of heat shock protein 72 (HSP72)were analyzed. Compared with Norm Sed, the relative content of HSP72 in the gastrocnemius in Heat Sed was significantly elevated (P < 0.05). After heat stress, the content of MDA in the gastrocnemius in Heat Exe significantly decreased (P < 0.05) and the content of SOD increased (P > 0.05) ,as compared with that in Norm Exe. These data demonstrate that heat stress respond before single-bout exercise can reduce the content of MDA in gastrocnemius.

关 键 词：热休克反应性 急性运动 骨骼肌 MDA SOD 脂质过氧化物 抗氧化物 

分 类 号：R873[医药卫生—运动医学]