血管内皮生长因子及其受体Flt-1在乳腺癌中的表达及意义  

Expression of vascular endothelial growth factor and its receptor (Flt 1) in breast carcinoma

作　　者：吴后男[1] 李玉林[1] 朱桂彬[1] 张丽红[1] 张秀英[1] 何旭[1] 

机构地区：[1]吉林大学基础医学院病理解剖教研室

出　　处：《中华医学杂志》2002年第10期708-711,共4页National Medical Journal of China

摘　　要：目的　试图揭示血管内皮生长因子 (VEGF)及其受体 (Flt 1)在乳腺癌组织中的表达规律 ,探讨其与血管生成和肿瘤浸润、转移等生物学行为的关系。方法　应用原位杂交和免疫组织化学技术 ,检测 4 8例乳腺癌组织中VEGF和Flt 1的mRNA和蛋白表达特性。结果　乳腺癌细胞高表达VEGFmRNA和蛋白 ,阳性率分别为 75 %、70 8% ,而血管内皮细胞几乎不表达 ;血管内皮细胞主要表达受体Flt 1mRNA和蛋白 ,阳性血管数分别为 2 6个 / 0 72mm2 ± 12个 / 0 72mm2 、2 4个 / 0 72mm2 ± 12个 / 0 72mm2 ,而少数癌细胞有少量表达 ;VEGF和Flt 1高表达组血管密度明显高于低表达组 ;VEGF和Flt 1表达与组织学分级和淋巴结转移密切相关。结论　VEGF以旁分泌途径促进乳腺癌血管形成 ,并参与肿瘤浸润和淋巴道转移过程 ,检测VEGF和Flt 1表达可做为判定乳腺癌血管生成、恶性度以及浸润转移等生物学行为的可靠指标。Objective To investigate the expression of vascular endothelial cell growth factor(VEGF)and its receptor (Flt 1) in breast carcinoma and the relationship of such expression with angiogenesis, tumor invision and metastasis. Methods In situ hybridyzation and immunohistochemistry technique were used to test the expression of mRNA and protein expression of VEGF and flt 1 in 48 specimens of breast carcinoma. 48 specimens of tissues near cancer, 3 speciens of fibroadenoma of breast, and 3 specimens of noemal breast were used as controls. Results VEGF mRNA and its protein were expressed highly in breast carcinoma cells with the positive rate of 75% and 70.8% respectively. There was almost no expression of VEGF in vascular endothelial cells. Flt 1 mRNA and its protein were expressed only in a small quantity in a few tumor cells. The amount of blood vessel positive in Fit 1 mRNA and its protein was (26±12 )piece/0.72 mm 2 and (24±12) piece/0.72 mm 2 respectively. The microvascular density (MVD) was significantly greater in VEGF and Flt 1 higher expression groups than in lower groups (P<0.01). Both VEGF expression and Flt 1 expression were well correlated with the histological grade and lymph node metastasis. Conclusion VEGF promotes angiogenesis by paracrining in breast carcinoma, and takes part in tumor invision and lymph node metastasis. Test of the expression of VEGF and Flt 1 may act as a reliable index to determine angiogenesis, malignance, invasion and metastasis. Blocking their secretion and effect may act as a new treatment for breast carcinoma.

关 键 词：血管内皮生长因子 受体FLT-1 乳腺癌 基因表达 临床意义 

分 类 号：R737.9[医药卫生—肿瘤]