利用基因工程菌去除电解废水中的汞离子  

Bioaccumulation of Mercury by Genetically Engineered Escherichia coli from Real Electrolytic Wastewater

作　　者：邓旭[1] 李清彪[1] 孙道华[1] 黄益丽[1] Wilson David B 

机构地区：[1]厦门大学化工系,福建厦门361005 [2]Dept.of Molecular Biology and Genetics

出　　处：《厦门大学学报（自然科学版）》2002年第3期330-333,共4页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金资助项目 (F0 10 0 0 0 8)

摘　　要：利用构建的基因工程菌生物富集真实电解废水中的汞离子 .电解废水中除含有 2 .5 8mg/L的汞离子外 ,还含有十种以上的其它成分 ,且 pH值为 9.6 .实验表明 ,与重组菌对只含汞离子的实验室模拟废水的处理结果比较 ,电解废水中其它组份的存在意外地增大了重组菌富集汞离子的作用速率 ,但同时却使细菌的最大汞富集量降低了30 % .废水 pH的变化对重组菌的汞富集行为影响很小 ,说明该基因工程菌能在很宽的pH范围内有效地富集汞 .实验还考察了EDTA及离子强度对富集行为的影响 .Bioaccumulation performance of mercury from real electrolyte wastewater by genetically engineered E. coli JM109, which expressed a mercurial ion transport system and metallothionein was investigated. These were more than 10 components existing in the wastewater besides 2.58 mg/L mercury, and the pH of the wastewater was 9.6. The co ions didn't decrease but somehow promoted mercury uptake rate of induced cells, while a 30% loss of the amount of mercury bioaccumulation was obtained in comparison with that from laboratory surrogate solution only containing mercury. The varying of pH in wastewater only slightly dropped mercury accumulation, suggesting induced cells had a property of pH insensitivity.

关 键 词：电解废水 基因工程菌 生物富集 废水处理 汞离子 废水组成 酸碱度 离子强度 

分 类 号：X781.103[环境科学与工程—环境工程] X172