尿样中苯骈二氮杂类药物筛选分析研究  

Screening of benzodizepines and their metabolites in urine

作　　者：沈保华[1] 沈敏[1] 卓先义[1] 赵子琴[2] 吴何坚[1] 卜俊[1] 

机构地区：[1]司法部司法鉴定科学技术研究所,上海200063 [2]复旦大学基础医学院法医教研室,上海200032

出　　处：《法医学杂志》2002年第1期22-25,共4页Journal of Forensic Medicine

基　　金：上海市科委基金资助项目(No.002512079)

摘　　要：目的建立尿样中苯骈二氮杂类药物两种筛选分析方法。方法用GC/ECD、GC/MS直接测定苯骈二氮杂类药物原体和GC/ECD、GC/MS测定1,4-苯骈二氮杂类药物的酸水解产物苯甲酮同系物。结果GC/ECD直接测定苯骈二氮杂类药物方法,大部分药物的回收率为60%～90%,线性范围为20～200ng/ml尿,线性相关系数大于0.99,最低检出限达0.5ng/ml～10ng/ml。结论所建两种方法各有其特点又可相互补充,已成功地应用于司法鉴定实践。Objective Screen seventeen benzodizepines and their metabolitesin urine by GC/ECDand GC/MS . Methods They were GC (GC/ECD, GC/MS) assay of benzodizepines and GC (GC/ECD, GC/MS) assay of benzophenones of acid-hydrolytic products of 1,4-benzodizepines. Results The methods were simple and sensitive. The recoverys were 60%to 90%of most benzodizepines, linear calibration curves were 20ng/ml-200ng/ml(r >0.99), and detection limits were 0.5ng/ml-10ng/ml. Conclusion They methods were evaluated with human urine samples.

关 键 词：苯骈二氮杂Zhu类药物 苯甲酮类同系物 GC/ECD GC/MS 法医物证检验学 

分 类 号：D919.2[医药卫生—法医学]