RT-PCR方法检测外周血CK-20在膀胱癌微转移灶的临床意义  

Clinical significance of micrometastasis of bladder carcinoma detected by CK-20 RT-PCR

作　　者：戴奇山[1] 钟惟德[1] 胡建波[1] 蔡岳斌[1] 魏鸿蔼[1] 高劲松[1] 刘健康[1] 刘洁珍[1] 肖聪勤[1] 曾广翘[1] 

机构地区：[1]广州市第一人民医院泌尿外科,广东510180

出　　处：《中国肿瘤临床与康复》2002年第3期46-48,共3页Chinese Journal of Clinical Oncology and Rehabilitation

基　　金：广东省科技厅重点立项 ( 2 0 0 138)

摘　　要：目的　研究膀胱癌微转移的情况及临床意义。方法　以靶向细胞角蛋白 2 0 (Cyokeratinck 2 0 )逆转录多聚酶链反应 (RT PCR)检查 91例膀胱癌微转移情况。结果　 91例膀胱癌中检出CK 2 0mRNA37例 ,阳性率 41% ,其中证实有转移的 2 0例中 17例阳性 ( 85 % ) ,发现无淋巴结转移的Ⅰ～Ⅱ期患者行TRRBP术后 15个月 2例复发者CK 2 0 mRNA均表达阳性 ;2 5例健康对照组均为阴性。结论CK 2 0RT PCR较常规组织学检查具有更高敏感性 ,提高了微转移的检出率 ,对判断患者预后 ,指导术后辅助治疗具有重要意义。Objective To study the micrometastasis of bladder carcinoma and its clinical importance.Methods 91 cases of bladder carcinoma were examined by cytokeratin 20 RT-PCR assay.Results Detection of CK-20 expression was positive in 37 of 91 patients with bladder carcinoma(41%).Among 20 patients with distant metastasis,17 were positive(85%),two recurrent cases in Ⅰ～Ⅱ stage with no lymphaden metastasis were positive 15 months after TURBT operation.CK-20 was not detected in blood samples from 25 normal individuals.Conclusion CK-20 RT-PCR is more sensitive than routine histology for the dectection of micrometastasis and more important in predicting the prognosis,the treatment after operation.

关 键 词：逆转录-聚合酶链反应 细胞角化蛋白20 膀胱癌 微转移 

分 类 号：R737.14[医药卫生—肿瘤]