β纤维蛋白原基因启动子区HaeⅢ多态性和血浆纤维蛋白原浓度与脑梗死的相关性研究  

The association between β HaeⅢ polymorphism located in the promoter region of β fibrinogen gene, plasma fibrinogen levels and cerebral infarction

作　　者：周盛年[1] 潘姝[1] 马玉燕[1] 迟兆富[1] 郭斌[1] 李大年[1] 

机构地区：[1]山东大学齐鲁医院神经内科,济南250012

出　　处：《脑与神经疾病杂志》2002年第3期134-136,共3页Journal of Brain and Nervous Diseases

摘　　要：目的 :研究中国人群中纤维蛋白原基因 β链的 Hae 限制性片段长度多态性与血纤维蛋白原水平和脑梗死 (CI)的关系。方法 :对 10 2名发病 10天内的脑梗死患者和 5 3名查体者用 PCR(聚合酶链反应 )和 RFL P(限制性片段长度多态性 )的方法 ,分析受试者的基因型 ,用 Follin酚法测定纤维蛋白原的浓度。统计学处理用 SPSS9.0软件。结果 :1CI患者携带 H1H2、H2 H2基因型频率较对照组明显增高 (P<0 .0 5 )。在 CI组 H2等位基因频率相对较高 (P<0 .0 5 )。 2βHae 多态性与血浆纤维蛋白原浓度间存在显著正相关 ,以βHae 酶切位点缺失的 H1H2 ,H2 H2基因型血浆纤维蛋白原浓度明显增高。结论 :脑梗死患者携带 H1H1、 H1H2基因型频率高 ,血浆纤维蛋白原浓度高 。Objective: To study the association between β HaeⅢ polymorphism located in the promoter region of β fibrinogen gene, plasma fibrinogen levels and cerebral infarction. Methods: Polymerase chain reaction (PCR) and restriction fragment length polymorphism(RFLP)were used to detect the fibrinogen genotype and plasma fibrinogen concentration was assayed with Follin phenol method in one hundred and two patients suffered from cerebral infarction within 10 days after the event and fifty three control subjects. Statietical analysis was carried out by SPSS 9.0 software. Results: ①The frequencies of the genotype H1H2, H2H2 were higher in patients with cerebral infarction than in control subjects P <0.05=②β HaeⅢ polymorphism are significantly associated with plasma fibrinogen levels P <0.05=The genotypes (H1H2, H2H2)which lost cutting site for HaeⅢ enzyme showed that plasma fibrinogen levels were higher in this groups. The frequencies of H2 allele were higher in patients with cerbral infarction. Conclusion: The afequencies of the genotype H1H2? H2H2 were higher in patients with cerbral infarction as compared with control subjects and plasma fibrinogen levels were significantly increased.

关 键 词：脑梗死 纤维蛋白原 βHaeⅢ多态性 

分 类 号：R743.330.2[医药卫生—神经病学与精神病学]