毫米波和阿霉素对K562细胞的影响  

Millimeter wave and adriamycin for purging leukemic cells in vitro

作　　者：孙桂玲[1] 江悦华[2] 赖声礼[1] 丁光生[1] 罗绍凯[3] 

机构地区：[1]华南理工大学电子与信息学院,广东广州510640 [2]广州军区广州总医院,广东广州510010 [3]中山医科大学,广东广州510080

出　　处：《中国医院药学杂志》2002年第6期325-327,共3页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金资助项目(60071033); 广东省自然科学基金资助项目(990587)

摘　　要：目的 :研究一种较好的毫米波和阿霉素的联合方法 ,用于白血病细胞的体外净化。方法 :将毫米波和阿霉素分别单独以及联合作用于K56 2细胞株 ,通过MTT测定细胞活力和流式细胞仪结合Annexin V/PI测定细胞的凋亡、坏死率来评价体外净化的效果。结果 :毫米波和阿霉素对细胞活力均表现出抑制作用 ,毫米波的抑制作用不呈现功率相关性 ,阿霉素的抑制程度呈现出浓度相关性 ;毫米波和阿霉素联合作用对K56 2细胞株的杀伤作用比单用阿霉素有显著性增加 (P <0 .0 1 )。结论 :毫米波和阿霉素联合作用于人白血病K56 2细胞能产生协同效应。OBJECTIVE To study the method of millimeter wave combined with adriamycin for purging leuk emic cells in vitro. METHODS K562 cell line was treated by millimeter wave and adriamycin alone or by the com bination of both. The inhibiton of cell growth was observed with MTT assay. Flow cytometre and Annexin-V/PI were used to analyse cell apoptosis and necroses. RESULTS Both millimeter wave and adriamycin showed an inhibitory effect on K562 cell. Th e inhibition of K562 cell line treated by millimeter wave and adriamycin was sig nificantly higher than that treated by adriamycin or millimeter wave alone ( P < 0.01 ).Compared with adriamycin alone, the combination of millimeter wave and adriamycin showed a remarkable increase killing effect on K562 cell line ( P < 0.01 ).CONCLUSIONS The combination of millimeter wave and adriamycin showed a synergistic effect on purging leukemic cells in vitro.

关 键 词：K562细胞 毫米波 阿霉素 白血病 ADR 

分 类 号：R733.7[医药卫生—肿瘤] R454.3[医药卫生—临床医学]