外周血甲胎蛋白mRNA定量与裸鼠肝癌术后复发转移的关系  

Quantitution of α-fetoprotein messenger RNA in peripheral blood of nude mice and its relationship with tumor re-currence and metastasis after curative resection of hepatocellular carcinoma

作　　者：吴晓凤[1] 林芷英[1] 樊嘉[1] 陆继珍[1] 王鲁[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所,上海200032

出　　处：《中华肝脏病杂志》2002年第3期189-192,共4页Chinese Journal of Hepatology

基　　金：上海市卫生系统百人计划基金(97BR029);上海市科技发展基金(984419067);上海市医学领先专业重点学科基金(983001)

摘　　要：目的 研究外周血中胎蛋白信使核糖核酸(AFP mRNA)水平与原发性肝癌根治性切除术后复发转移的关系。方法 裸鼠肝内原位接种裸鼠人肝癌转移模型LCI-D20肿瘤组织,接种后第10天切除移植瘤,切除后第2天用不同剂量干扰素α-1b(IFNα-1b )治疗,治疗35 d后取外周血1 ml,用TaqMan实时定量逆转录－聚合酶链反应(RT-PCR)技术检测AFP mRNA水平,同时观察肿瘤复发转移情况。结果 对照组裸鼠术后肝内复发率和肺转移率均为100%(12/12),外周血AFP mRNA阳性率为100%。小剂量IFNα-1b治疗组肝内复发率62.5%(5/8),复发瘤体积小于对照组(25 mm^3±2mm^3对1143mm^3±3mm^3,t=9.27,P＜0.01),无肺转移,外周血AFP mRNA阳性率87.5%(7/8),水平低于对照组[(85±6)copies/μg对(955±2)copies/μg,t＝4.33,P＜0.01)。大剂量IFNα-1b治疗组肝内复发率为12.5%(1/8),体积仅0.5mm~,无肺转移,外周血AFP mRNA均阴性(x^2=11.67,P＜0.01)。结论 外周血AFP mRNA可作反映肝癌复发转移的敏感指标,TaqMan实时定量RT-PCR技术检测循环血肝癌细胞灵敏、简便、精确度高。ve To assess the level of α-fetoprotein (AFP) messenger RNA (mRNA) in peripheral blood ofnude mice, and to study its relationship with tumor recurrence and metastasis after curative resection of hepatocellular carci-noma (HCC). Methods The metastatic model of human HCC in nude mice LCI-D20 was used in this study. Curativeresection was performed at l0th day after tumor implantation in 28 nude mice. Interferon α- lb (IFNα-lb) was adminis-tered subcutaneously from the next day after resection, at doses of 3×l0~7 U·kg^(-1)·d^(-l) (8 nude mice), l.5×l0~7U· kg^(-1)·d^(-l) (8 nudemice) respectively in the beatment groups, and normal saline alone in the controlled group (l2 nude mice). Thirty-five days aftertreament. one millilier of peripheral bloed was taken and AFP mRNA was quantitatively analyzed using TaqMan real-time quan-titative RT-PCR. The mice were sacrificed. The size of recurrent tumor was measured and the presence of lung metastases wasobserved. Results The liver recurrent rate, lung metastatic rate and positivity of AFP mRNA in the controlled group were alll00% (l2/l2), whereas it was 62.5% (5l8), 0% (0/8), 87.5% (7/8) respectively in the IFN α-lb l.5 ×l0~7U·kg^(-l)·d^(-l) treated group.The recurrent tumor in liver of the IFN α-lb l.5×l0~7 U·kg^(-1)·d^(-l) treated group was much smaller than that of the controlled group(25 mm^3±2 mm^3 vs l l43 mm^3±3 mm^3, t = 9.27, P＜0.0l). and the level of AFP mRNA was also lower than that of the controlledgroup [(85±6)copies/μg vs (955±2) copies/μg, t = 4.33, P＜0.0l]. In the IFN α-lb 3×l0~7 U·kg^(-1)·d^(-1)treated group, there wasonly one recurrent tumor(0.5 mm^3), no lung metastasis, and the positivity of AFP mRNA was 0% (x^2 =l1.67, P＜0.0l ). ConclusionsThese results suggest that the level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection ofHCC. TaqMan real the quantitative RT-PCR is a very sensitive and convenient method for detecting circulating cancer cells.

关 键 词：肝癌 术后 甲胎蛋白 肿瘤转移 信使核糖核酸 肿瘤复发 

分 类 号：R735.7[医药卫生—肿瘤]