抗血管内皮生长因子发卡状核酶基因抑制卵巢癌细胞SKOV3增殖  

Proliferation of ovarian cancer cell SKOV3 were inhibited by Anti-VEGF hairpin ribozyme gene

作　　者：严瑞兰[1] 钱新华[1] 辛晓燕[2] 金明[3] 惠宏襄[3] 王健[2] 王德堂[2] 

机构地区：[1]第一军医大学南方医院小儿科,广州510515 [2]第四军医大学西京医院 [3]第四军医大学西京医院分子生物学教研室

出　　处：《肿瘤防治研究》2002年第3期168-169,172,共3页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目 (3970 0 14 7)

摘　　要：目的　观察抗VEGF发卡状核酶基因对卵巢癌细胞增殖的影响。方法　采用脂质体介导的方法将自行设计和构建的抗VEGF发卡状核酶基因真核表达载体转染人卵巢癌细胞 ,通过MTT、细胞贴壁克隆形成试验、软琼脂克隆形成试验及细胞周期分析观察其对卵巢癌细胞增殖的影响。结果　转染后的SKOV3 RZ细胞生长减慢 ,细胞贴壁克隆形成及软琼脂克隆形成率明显降低 (P <0 .0 0 1)。G1期细胞显著增多 (P <0 .0 1) ,S期细胞显著减少 (P <0 .0 1)。结论　抗VEGF发卡状核酶基因可显著抑制卵巢癌细胞SKOV3增殖 。Objectivc To observe the effect of Anti\|VEGF hairpin ribozyme gene to ovarian cancer cells. Methods Anti\|VEGF hairpin ribozyme gene eukaryotic expression vector was introduced into ovarian cancer SKOV3 cells by using lipofectin mediation; MTT, colony forming examination, soft agar colony forming examination and FCM were used to observe the effect of proliferation to ovarian cancer cells. Results The growth of transfected SKOV3\|RZ cells were slower; The average colony forming efficiency and soft agar colony forming efficinecy of SKOV3\|RZ cells were distinctly decreased( P <0.001); The SKOV3\|RZ cells of G1 stage were increased( P <0.01), the SKOV3\|RZ cells of S stage were reduced( P <0.01).Conclusion Anti\|VEGF hairpin ribozyme gene can inhibit the proliferation of ovarian cancer SKOV3 cells. This provides a expermental basis for cure human ovarian cancer with antiangiogenesis method.

关 键 词：抗血管内皮生长因子 SKOV3增殖 发卡状核酶基因 卵巢癌 细胞增殖 

分 类 号：R737.31[医药卫生—肿瘤]