胰腺癌伴淋巴结转移的基因芯片研究  

cDNA microarray in the gene expression pattern in lymphatic metastasis of pancreatic cancinoma

作　　者：谭志军[1] 胡先贵[1] 应康[2] 李瑶[2] 唐榕[2] 曹贵松[1] 唐岩[1] 金钢[1] 

机构地区：[1]长海医院普外科,上海200433 [2]复旦大学生命科学院遗传研究所

出　　处：《中华肿瘤杂志》2002年第3期243-246,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目 ( 30 0 0 0 16 0 )

摘　　要：目的　利用基因芯片技术分析伴与不伴淋巴结转移胰腺癌组织中的差异表达基因。方法　将 40 0 0个人类全长基因的PCR产物点样于特殊处理后的玻片上制备基因芯片。用逆转录的方法 ,分别将两种荧光Cy3 dUTP和Cy5 dUTP标记正常胰腺组织和胰腺癌组织mRNA ,以制备cDNA探针 ,并与芯片杂交。以ScanArray 30 0 0荧光扫描仪扫描芯片上两种荧光信号 ,获得的荧光信号图像用计算机分析。每点上两种荧光信号的强度分别代表Cy3 dUTP和Cy5 dUTP的量。最后 ,计算每点的Cy5和Cy3比值。结果　在所检测的 2例伴淋巴结转移和 2例不伴淋巴结转移的胰腺癌标本中 ,5 6个基因 (其中包括 2 4个已知基因 )有表达差异。结论　基因芯片技术为阐明人类胰腺癌伴淋巴结转移的特异性基因表达提供了有效方法。Objective Analysis of differential gene expression profiles by cDNA microarray in pancreatic carcinoma with or without lymphatic metastasis.Methods cDNA microarray was prepared by spotting prolymerase chain reaction (PCR) products of 4 000 human genes onto specially treated glass slides. The cDNA probes were prepared by labeling normal tissue mRNA and cancer tissue mRNA with Cy3-dUTP and Cy5-dUTP, separately through reverse transcription. The mixed probes were, then, hybridized to the cDNA microarray. The chips were scanned by ScanArray 3000 laser scanner (General Scanning, Inc) on two wavelengths. The acquired image was analyzed by ImaGene 3.0 software (BioDiscovery, Inc). The intensity of each spot on the two wavelengths represented the quantity of Cy3-dUTP and Cy5-dUTP, with Cy5 to Cy3 ratio computed on each. Results Fifty-six genes (including 24 preiously reported) exhibited differential expressions in 2 specimens of pancreatic carcinoma with lymphatic metastasis and 2 without. Conclusion cDNA microarray provides an promising approach to specific gene expressions of the presence of lymphatic metastasis in human pancreatic carcinoma.

关 键 词：胰腺癌 淋巴结转移 基因芯片 研究 胰腺肿瘤 淋巴转移 

分 类 号：R735.9[医药卫生—肿瘤] R733.4[医药卫生—临床医学]