硅胶键合手性配体交换色谱固定相键合量对α-氨基酸拆分的影响  

Effect of the Bonding Amounts of Chiral Ligand Exchange Chromatogr-aphic Stationary Phases on the Enantioseparation of Amino Acids

作　　者：祝馨怡[1] 韩小茜[1] 齐邦峰[1] 李永民[1] 蒋生祥[1] 陈立仁[1] 

机构地区：[1]中国科学院兰州化学物理研究所,甘肃兰州730000

出　　处：《色谱》2002年第3期223-226,共4页Chinese Journal of Chromatography

基　　金：中国科学院重点基金资助项目 (批准号 :KY95 S1 2 0 5 )

摘　　要：合成了两种不同键合量的L 脯氨酸硅胶键合手性配体交换固定相 ,装柱后利用配体交换色谱法分离了一系列的α 氨基酸。实验结果表明键合量不同的固定相对α 氨基酸的拆分能力差别较大。L proline chiral stationary phases with various bonding amounts for ligand exchange chromatography were prepared by treating silica gel with 3 glycidoxypropyltrimethoxysilane and bonding L proline. The bonding amounts of L proline chiral stationary phase were controlled effectively and characterized by IR and elemental analysis. Some DL amino acids were separated and the influences of the bonding amounts on the efficiency of resolution of DL amino acids by ligand exchange chromatography were briefly discussed and explained by the mechanism of the separation.

关 键 词：硅胶键合手性配体 配体交换色谱 L-脯氨酸 手性固定相 对映体分离 键合量 Α-氨基酸 手性拆分 

分 类 号：O657.7[理学—分析化学] O629.711[理学—化学]